LOCUS       sequence-158617-        5105 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for abrB1941(CAC1941) overexpression in Clostridium 
            acetobutylicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y
  TITLE     Roles of three AbrBs in regulating two-phase Clostridium 
            acetobutylicum fermentation.
  JOURNAL   Appl Microbiol Biotechnol. 2016 Jun 8.
  PUBMED    27276910
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2016 Jun 8."
COMMENT     Sequence Label: pIMP1-Pthl-AbrB1941
FEATURES             Location/Qualifiers
     source          1..5105
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             408..416
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(446..463)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(446..462)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(455..477)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(671..690)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     790..812
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(850..868)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        936..1040
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1041..1109,1110..1901)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1259..1278)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2072..2660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2561..2580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2814..2831
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2948..2969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2984..2989,2990..3007,3008..3014)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3022..3038
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3027..3049
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3046..3062
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3046..3062
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttggctg cagtttttaa caaaatatat tgataaaaat aataatagtg ggtataatta
       61 agttgttaga gaaaacgtat aaattaggga taaactatgg aacttatgaa atagattgaa
      121 atggtttatc tgttaccccg tatcaaaatt taggaggtta gttagagtcg actctagaat
      181 gaaatcaaca ggaatagtaa gaaaattgga tagcttggga agggtagtaa tacctaaaga
      241 attgagaaga acatttaata tagcagaaaa ggacgcttta gaaatttatg tagatggtga
      301 gcagatagtc ttaaagaaat ataatccagc atgtgtgttc tgcggtgaag caagcgacgt
      361 aataaattat aaaggaaaga agatatgtaa gaagtgcttg aaagaattaa ggaggtaggg
      421 atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
      481 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
      541 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
      601 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
      661 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
      721 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
      781 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
      841 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
      901 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     1081 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     1141 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     1201 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     1261 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     1321 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     1381 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     1441 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     1501 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     1561 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     1621 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     1681 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     1741 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     1801 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1861 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1921 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1981 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2041 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2101 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2161 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2221 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2281 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2341 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2401 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2461 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     2521 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     2581 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     2641 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     2701 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     2761 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     2821 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2881 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2941 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3001 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3061 accatgatta cgccaagctt tggctaacac acacgccatt ccaaccaata gttttctcgg
     3121 cataaagcca tgctctgacg cttaaatgca ctaatgcctt aaaaaaacat taaagtctaa
     3181 cacactagac ttatttactt cgtaattaag tcgttaaacc gtgtgctcta cgaccaaaag
     3241 tataaaacct ttaagaactt tcttttttct tgtaaaaaaa gaaactagat aaatctctca
     3301 tatcttttat tcaataatcg catcagattg cagtataaat ttaacgatca ctcatcatgt
     3361 tcatatttat cagagctcgt gctataatta tactaatttt ataaggagga aaaaataaag
     3421 agggttataa tgaacgagaa aaatataaaa cacagtcaaa actttattac ttcaaaacat
     3481 aatatagata aaataatgac aaatataaga ttaaatgaac atgataatat ctttgaaatc
     3541 ggctcaggaa aagggcattt tacccttgaa ttagtacaga ggtgtaattt cgtaactgcc
     3601 attgaaatag accataaatt atgcaaaact acagaaaata aacttgttga tcacgataat
     3661 ttccaagttt taaacaagga tatattgcag tttaaatttc ctaaaaacca atcctataaa
     3721 atatttggta atatacctta taacataagt acggatataa tacgcaaaat tgtttttgat
     3781 agtatagctg atgagattta tttaatcgtg gaatacgggt ttgctaaaag attattaaat
     3841 acaaaacgct cattggcatt atttttaatg gcagaagttg atatttctat attaagtatg
     3901 gttccaagag aatattttca tcctaaacct aaagtgaata gctcacttat cagattaaat
     3961 agaaaaaaat caagaatatc acacaaagat aaacagaagt ataattattt cgttatgaaa
     4021 tgggttaaca aagaatacaa gaaaatattt acaaaaaatc aatttaacaa ttccttaaaa
     4081 catgcaggaa ttgacgattt aaacaatatt agctttgaac aattcttatc tcttttcaat
     4141 agctataaat tatttaataa gtaagttaag ggatgcataa actgcatccc ttaacttgtt
     4201 tttcgtgtac ctattttttg tgaatcgatt atgtcttttg cgcattcact tcttttctat
     4261 ataaatatga gcgaagcgaa taagcgtcgg aaaagcagca aaaagtttcc tttttgctgt
     4321 tggagcatgg gggttcaggg ggtgcagtat ctgacgtcaa tgccgagcga aagcgagccg
     4381 aagggtagca tttacgttag ataaccccct gatatgctcc gacgctttat atagaaaaga
     4441 agattcaact aggtaaaatc ttaatatagg ttgagatgat aaggtttata aggaatttgt
     4501 ttgttctaat ttttcactca ttttgttcta atttctttta acaaatgttc tttttttttt
     4561 agaacagtta tgatatagtt agaatagttt aaaataagga gtgagaaaaa gatgaaagaa
     4621 agatatggaa cagtctataa aggctctcag aggctcatag acgaagaaag tggagaagtc
     4681 atagaggtag acaagttata ccgtaaacaa acgtctggta acttcgtaaa ggcatatata
     4741 gtgcaattaa taagtatgtt agatatgatt ggcggaaaaa aacttaaaat cgttaactat
     4801 atcctagata atgtccactt aagtaacaat acaatgatag ctacaacaag agaaatagca
     4861 aaagctacag gaacaagtct acaaacagta ataacaacac ttaaaatctt agaagaagga
     4921 aatattataa aaagaaaaac tggagtatta atgttaaacc ctgaactact aatgagaggc
     4981 gacgaccaaa aacaaaaata cctcttactc gaatttggga actttgagca agaggcaaat
     5041 gaaatagatt gacctcccaa taacaccacg tagttattgg gaggtcaatc tatgaaatgc
     5101 gatta
//
