LOCUS       sequence-158619-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for abrB3647(CAC3647) overexpression in Clostridium 
            acetobutylicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y
  TITLE     Roles of three AbrBs in regulating two-phase Clostridium 
            acetobutylicum fermentation.
  JOURNAL   Appl Microbiol Biotechnol. 2016 Jun 8.
  PUBMED    27276910
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2016 Jun 8."
COMMENT     Sequence Label: pIMP1-Pthl-AbrB3647
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     216..233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    350..371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(386..391,392..409,410..416)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    424..440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     429..451
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     448..464
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     448..464
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      241 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      301 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      361 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      421 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      481 tttggctaac acacacgcca ttccaaccaa tagttttctc ggcataaagc catgctctga
      541 cgcttaaatg cactaatgcc ttaaaaaaac attaaagtct aacacactag acttatttac
      601 ttcgtaatta agtcgttaaa ccgtgtgctc tacgaccaaa agtataaaac ctttaagaac
      661 tttctttttt cttgtaaaaa aagaaactag ataaatctct catatctttt attcaataat
      721 cgcatcagat tgcagtataa atttaacgat cactcatcat gttcatattt atcagagctc
      781 gtgctataat tatactaatt ttataaggag gaaaaaataa agagggttat aatgaacgag
      841 aaaaatataa aacacagtca aaactttatt acttcaaaac ataatataga taaaataatg
      901 acaaatataa gattaaatga acatgataat atctttgaaa tcggctcagg aaaagggcat
      961 tttacccttg aattagtaca gaggtgtaat ttcgtaactg ccattgaaat agacca
//
