LOCUS       sequence-158628-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For tagging mitochondria with Myc epitopes (Control-MITO construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Chen WW, Freinkman E, Wang T, Birsoy K, Sabatini DM
  TITLE     Absolute Quantification of Matrix Metabolites Reveals the Dynamics 
            of Mitochondrial Metabolism.
  JOURNAL   Cell. 2016 Aug 25;166(5):1324-1337.e11. doi: 
            10.1016/j.cell.2016.07.040.
  PUBMED    27565352
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.07"; journalName: "Cell"; date: "2016-08-25- 
            25"; volume: "166"; issue: "5"; pages: "1324-1337.e11"
COMMENT     Sequence Label: pMXs-3XMyc-EGFP-OMP25
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             98..127
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             140..169
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             182..211
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(224..226,227..937)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(224..226,227..937)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(266..287)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(527..546)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     874..895
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gggggtggac catcctctag actgccggat ctagctagtt aattaaggat cccagtgtgg
       61 tggtacggga attcctgcag gcctcgaggc caccatggag cagaagctga tttctgagga
      121 agatctgggc acaggatccg aacagaaact gatttctgag gaagatctgg gcagcgccgg
      181 agagcagaag ctgatttctg aagaggatct gggagggagc ggcgtgagca agggcgagga
      241 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
      301 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
      361 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
      421 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
      481 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
      541 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
      601 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
      661 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
      721 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
      781 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
      841 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
      901 cgccgggatc actctcggca tggacgagct gtacaagccg cggcatcgag gcgacggaga
      961 gcctagtgga gttcctgtag ctgtggtgct gctgccagtg tttgccctta ccctggtagc
     1021 agtttgggcc ttcgtgagat accgaaagca gctctgagcg gccgc
//
