LOCUS       sequence-158645-       11536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Gateway cloning of enhancer elements upstream of the DSCP and 
            GFP::Luciferase(nls) reporter, facilitating qualitative and 
            quantitative analysis of expression in transgenic fly lines and S2 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11536)
  AUTHORS   Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, 
            Harrison MM, Lee CY
  TITLE     An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and 
            Rapid Restriction of Developmental Potential during Asymmetric Stem 
            Cell Division.
  JOURNAL   Dev Cell. 2017 Feb 27;40(4):367-380.e7. doi: 
            10.1016/j.devcel.2017.01.014.
  PUBMED    28245922
REFERENCE   2  (bases 1 to 11536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.01"; journalName: "Dev Cell"; date: 
            "2017-02-27- 27"; volume: "40"; issue: "4"; pages: "367-380.e7"
COMMENT     Sequence Label: VanGlow-GL-DSCP
FEATURES             Location/Qualifiers
     source          1..11536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2052..2054,2055..2057,2058..2768)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5031..5047)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5031..5047)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(6058..6672,6743..6874,7078..7393,7455..8109,
                     8184..8457,8859..8930))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9382..9400
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9625..9694
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9781..10369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9861..9880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10540..11331,11332..11400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11163..11182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11401..11505)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accagatccg ggatcccgta
     1981 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagcccaagc ttgaagcaag
     2041 cctcctgcaa catggtgtcc aagggcgagg agctgttcac cggcgtggtg ccaattctgg
     2101 tggagctgga tggcgacgtg aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     2161 acgccaccta tggaaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     2221 catggccaac cctcgtgacc accctgacct atggcgtgca gtgcttcagc cgctaccccg
     2281 atcacatgaa gcagcacgat ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc
     2341 gcaccatctt tttcaaggat gacggcaact acaagacccg cgccgaagtg aagttcgagg
     2401 gcgataccct cgtgaaccgc atcgagctga agggcatcga tttcaaggag gatggaaaca
     2461 tcctgggcca caagctggag tacaactaca acagccacaa cgtgtacatc atggccgaca
     2521 agcagaagaa cggcatcaaa gtgaacttca agatccgcca caacatcgag gatggcagcg
     2581 tgcagctggc cgatcactac cagcagaaca ccccaatcgg cgacggccca gtgctgctgc
     2641 ccgataacca ttacctgagc acccagagcg ccctgagcaa ggatcccaac gagaagcgcg
     2701 accacatggt gctgctggag tttgtgaccg ccgccggcat taccctgggc atggatgagc
     2761 tgtacaagaa gaacatcaag aagggcccag ccccgttcta ccccctggag gacggaaccg
     2821 ccggagagca gctgcacaag gccatgaagc gctacgccct ggtgcccggc acgatcgcct
     2881 ttaccgatgc ccacatcgag gtggacatca cctacgccga gtacttcgag atgagcgtgc
     2941 gcctggccga ggccatgaag cgctatggcc tgaacaccaa ccaccgcatc gtcgtgtgca
     3001 gcgagaacag cctgcagttc ttcatgcccg tgctgggcgc cctgttcatt ggagtggccg
     3061 tggccccagc caacgatatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     3121 agcccaccgt ggtgttcgtg tccaagaagg gactgcagaa gatcctgaac gtgcagaaga
     3181 agctgcccat catccagaag atcatcatca tggacagcaa gaccgactac cagggcttcc
     3241 agagcatgta caccttcgtg accagccatc tgccacccgg cttcaacgag tacgatttcg
     3301 tgcccgagag cttcgatcgc gataagacca ttgccctgat catgaacagc agcggcagca
     3361 ccggactgcc aaagggcgtg gccctgccac accgcaccgc ctgcgtgcgc tttagtcatg
     3421 cccgcgatcc catcttcggc aaccagatca tccccgatac cgccatcctg tccgtggtgc
     3481 ccttccacca cggcttcggc atgttcacca ccctgggcta cctgatctgc ggcttccgcg
     3541 tggtgctgat gtaccgcttc gaggaggagc tgtttctgcg cagcctgcag gattacaaga
     3601 tccagtccgc cctgctggtg cccaccctgt tctcgttctt cgccaagagc accctgatcg
     3661 ataagtacga tctgagcaac ctgcacgaga tcgccagcgg cggagcccca ctgtccaagg
     3721 aagtgggcga ggccgtggcc aagcgctttc atctgcccgg aattcgccag ggctacggcc
     3781 tgaccgagac caccagcgcc attctgatca ccccagaggg cgacgataag ccaggcgccg
     3841 tgggaaaggt ggtgccgttt ttcgaggcca aggtggtgga tctggacacg ggcaagaccc
     3901 tgggagtgaa tcagcgcgga gagctgtgcg tgcgcggacc gatgattatg agcggctacg
     3961 tgaacaaccc cgaggccacc aacgccctga ttgacaagga tggctggctg cacagcggcg
     4021 atattgccta ctgggatgag gatgagcact tcttcatcgt ggatcgcctg aagtccctga
     4081 tcaagtacaa gggctaccag gtggccccag ccgagctgga gagtatcctg ctgcagcacc
     4141 ccaacatctt cgatgccggc gtggccggcc tgcccgatga tgacgccggc gagctgccag
     4201 ccgccgtggt ggtgctggag catggaaaga ccatgaccga gaaggagatc gtggattacg
     4261 tggccagcca agtgaccacc gccaagaagc tgcgcggagg cgtggtgttt gtggatgagg
     4321 tgcccaaggg cctgacgggc aagctggatg cccgcaagat tcgcgagatc ctgattaagg
     4381 ccaagaaggg cggcaagatc gccgtgtccc gccaccgccg ccatcgccag cgcagtcgca
     4441 gccgaaatcg cagtcgatcc cgaagtagcg agcgaaagcg acgacagcgc tcccgctcgc
     4501 gcagttccga gcgtcgacgc tacctgtaca agtagaatga atcgtagata ctgaaaaacc
     4561 ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg
     4621 ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct
     4681 gatctataga attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt
     4741 cttcatgaaa atatattacg agggcttatt cagaagctta tcgataccgt cgactaaagc
     4801 caaatagaaa attattcagt tcctggctta agtttttaaa agtgatatta tttatttggt
     4861 tgtaaccaac caaaagaatg taaataacta atacataatt atgttagttt taagttagca
     4921 acaaattgat tttagctata ttagctactt ggttaataaa tagaatatat ttatttaaag
     4981 ataattgcgt ttttattgtc agggagtgag tttgcttaaa aactcgttta gatccactag
     5041 ttctagagcg gccgctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     5101 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
     5161 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     5221 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
     5281 aggcgcgccc tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat
     5341 tatagttgtt atttttgata tatataaaca acactattat gcccaccatt tttttgagat
     5401 gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca
     5461 ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat
     5521 agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt
     5581 attactaatt tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg
     5641 cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga
     5701 gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga
     5761 agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc
     5821 gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa
     5881 tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc
     5941 ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa
     6001 cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta
     6061 ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat
     6121 gagaatggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc
     6181 caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc
     6241 cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga
     6301 gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc
     6361 aaagagcagg aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga
     6421 gctggcgcag gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag
     6481 cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat
     6541 cgccgtgaag accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag
     6601 aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc
     6661 tgaggtgaac acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa
     6721 atgtattcta aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg
     6781 tcaggaagag gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc
     6841 ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc
     6901 tcattaatat tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac
     6961 atctcattga aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg
     7021 aatttgtttc caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt
     7081 tgtctgaata agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg
     7141 ccacaggacc gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg
     7201 ctgctccagt ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac
     7261 tttgctaatg gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg
     7321 tccgggcaca acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca
     7381 ctgggcaccc acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa
     7441 cacatcgaac tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta
     7501 ccctgccctc ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct
     7561 gatgaatggt caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga
     7621 cgctgtgggc ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg
     7681 gatcggttag agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt
     7741 tcaccctgcc gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga
     7801 tcacctgatc cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca
     7861 tggcttggaa aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct
     7921 ggacataggc gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc
     7981 gcatcccgga tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca
     8041 gggtcgtctt tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca
     8101 cgccgcaaac tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca
     8161 gccgactgcg aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg
     8221 tcgctcgttg cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg
     8281 ctgattgacc gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag
     8341 ttggccgctc cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt
     8401 tttggcctgc ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg
     8461 caatgatagg ggatctcaaa tatcaactac tagcgttatg ctcatctaac cccgaacaaa
     8521 aagtaccccg aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta
     8581 ggatcagctt aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg
     8641 aatgccttta gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac
     8701 agctaccaga ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt
     8761 ttgtgggtcg cagttctcac gccgcataca atttgatgtt gcaatcgcag ttcctataga
     8821 tcaagtgaac ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat
     8881 gggtgtttgc tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt
     8941 tttcacactt tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa
     9001 caaacaaaaa taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg
     9061 taaaccgctt ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg
     9121 acgctacgta acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac
     9181 ggcgatattt ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat
     9241 tttgagtgag aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat
     9301 ttaaaactga tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt
     9361 ttatctactt tccgcaaaaa tgggttttat taacttacat acatactagg cgcgcccata
     9421 tgttcggctt gtcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt
     9481 atccgcaccc gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg
     9541 tttcgagggc gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact accgccacct
     9601 cgacccgttc atcatgatgg accagatggg tgaggtggag tacgcgcccg gggagcccaa
     9661 gggcacgccc tggcacccgc accgcggctt cgagaccgtg acctacatcg tcgacggtaa
     9721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    10021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    10081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    10141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    10201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    10261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    11041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    11101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    11161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    11221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    11281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11521 agtgccacct gacgtc
//
