LOCUS       sequence-158651-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23109_tetR PEZ1_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ04
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             57..68
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(77..111)
                     /label=BBa_J23109
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.04"
ORIGIN
        1 agcagctcta atgcgctgtt aatcacttta cttttatcta atctggacat ctagtatttc
       61 tcctctttct ctagtagcta gcacagtccc taggactgag ctagctgtaa aggaattctt
      121 aacaaaatcc ctatcagtga tagaacaaga tgtaacatcc ctatcagtga tagagtataa
      181 agttaactgt gggattgcaa aaggagctcc caactcataa agtcaagtag gagattaatc
      241 atatgtcggt catcaagccc gaaatgaaaa ttaaactgcg catggagggc gctgtgaacg
      301 ggcacaagtt cgtgatcgaa ggtgaaggca ttggtaagcc gtatgaaggt actcaaaccc
      361 tggacttaac ggttaaagaa ggtgccccgt tgccatttag ctacgacatc ctgacacccg
      421 cttttcagta tggcaatcgc gcgttcacca agtatccgaa agatattccg gattatttca
      481 agcaggcatt cccggaagga tactcatggg aacgctcgat gacctacgaa gaccagggca
      541 tttgcattgc cacaagcgac atcacaatgg agggtgattg ctttttttac aaaattcgct
      601 tcgacgggac aaacttcccg ccgaatggcc cagtgatgca gaaaaagacg cttaaatggg
      661 aaccgtcgac agagaaaatg tacgttcgcg atggcgttct gaaaggcgat gttaatatgg
      721 cgctgcttct cgaaggggga ggccattacc gttgtgattt caaaaccaca tacaaagcaa
      781 aaaaagatgt tcgtttgccg gacgcgcatg aagtcgatca ccgcattgag atcctgt
//
