LOCUS       sequence-158654-       10876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Gateway cloning of enhancer elements upstream of the DSCP and 
            mCherry::Renilla(nls) reporter, facilitating qualitative and 
            quantitative analysis of expression in transgenic fly lines and S2 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, 
            Harrison MM, Lee CY
  TITLE     An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and 
            Rapid Restriction of Developmental Potential during Asymmetric Stem 
            Cell Division.
  JOURNAL   Dev Cell. 2017 Feb 27;40(4):367-380.e7. doi: 
            10.1016/j.devcel.2017.01.014.
  PUBMED    28245922
REFERENCE   2  (bases 1 to 10876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.01"; journalName: "Dev Cell"; date: 
            "2017-02-27- 27"; volume: "40"; issue: "4"; pages: "367-380.e7"
COMMENT     Sequence Label: VanGlow-RR-DSCP
FEATURES             Location/Qualifiers
     source          1..10876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4371..4387)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4371..4387)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5398..6012,6083..6214,6418..6733,6795..7449,
                     7524..7797,8199..8270))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8722..8740
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8965..9034
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9121..9709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9201..9220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9880..10671,10672..10740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10503..10522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10741..10845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accagatccg ggatcccgta
     1981 agcaaataaa caagcgcagc tgaacaagct aaacaatctg cagcccaagc ttgaagcaag
     2041 cctcctgcaa catggtgtcc aagggcgagg aggataacat ggccatcatc aaggagttca
     2101 tgcgcttcaa ggtgcacatg gagggcagcg tgaacggcca cgagttcgag attgagggcg
     2161 agggcgaggg acgcccatat gagggaaccc agaccgccaa gctgaaagtg accaagggcg
     2221 gacccctgcc cttcgcctgg gatattctga gcccccagtt tatgtacggc agcaaggcct
     2281 acgtgaagca ccccgccgat atccccgatt acctgaagct gagcttcccc gagggcttca
     2341 agtgggagcg cgtgatgaac ttcgaggatg gcggcgtcgt gaccgtgacc caggatagca
     2401 gtctgcagga tggcgagttc atctacaaag tgaagctgcg cggcaccaac ttccccagcg
     2461 acggaccagt gatgcagaag aagaccatgg gctgggaggc cagcagcgag cgcatgtatc
     2521 cagaggacgg cgccctgaag ggcgagatca agcagcgcct gaagctgaag gatggcggcc
     2581 actacgatgc cgaagtgaag accacctaca aggccaagaa gcccgtgcag ctgccaggcg
     2641 cctacaacgt gaacatcaag ctggatatca ccagccacaa cgaggactac accatcgtgg
     2701 agcagtacga gcgcgccgag ggccgccata gtaccggcgg aatggatgag ctgtacaagg
     2761 gcaccagcgg cagcggaagt ggaagcggat ccggcagtgg atccggaagt gcctgcacca
     2821 gcaaggtgta cgatcccgag cagcgcaagc gcatgatcac cggaccacag tggtgggccc
     2881 gctgcaagca gatgaacgtg ctggatagct tcatcaacta ctacgacagc gagaagcacg
     2941 ccgagaacgc cgtgatcttc ctgcacggaa atgccgccag cagctacctg tggcgccatg
     3001 tggtgccaca catcgagcca gtggcccgct gcatcatccc cgatctgatc ggcatgggca
     3061 agagcggcaa gtccggcaat ggaagctacc gcctgctgga tcactacaag tacctgaccg
     3121 cctggttcga gctgctgaac ctgcccaaga agatcatttt cgtgggccac gattggggcg
     3181 cctgcctggc cttccattac agctacgagc accaggataa gatcaaggcc atcgtgcatg
     3241 ccgagagcgt ggtggatgtg atcgagagct gggatgagtg gcccgatatc gaggaggaca
     3301 ttgccctgat caagtccgag gagggcgaga agatggtgct ggagaacaac ttcttcgtgg
     3361 agaccatgct gccgagcaag attatgcgca agctggagcc cgaggagttc gccgcctatc
     3421 tggagccctt caaggagaag ggcgaagtgc gccgcccaac cctgagttgg ccacgcgaga
     3481 ttccactcgt gaagggcggc aagcccgatg tggtgcagat cgtgcgcaac tacaacgcct
     3541 acctgcgcgc ctccgatgat ctgccgaaga tgttcatcga gagcgatccc ggcttcttca
     3601 gcaacgccat tgtggagggc gccaagaagt tccccaacac cgagttcgtg aaagtgaagg
     3661 gcctgcactt cagccaggag gatgccccag acgagatggg caagtacatc aagtcgttcg
     3721 tggagcgcgt gctgaagaac gagcagagtc gccatcgccg ccaccgccag cgcagtcgca
     3781 gccgcaatcg cagtcgctcc cgcagtagcg agcgcaagcg tcgacagcgc tcccgcagcc
     3841 gaagttccga gcgtcgacgc tacctgtaca agtagaatga atcgtagata ctgaaaaacc
     3901 ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat ttatacatcg
     3961 ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca tgtaacctct
     4021 gatctataga attttttaaa tgactagaat taatgcccat cttttttttg gacctaaatt
     4081 cttcatgaaa atatattacg agggcttatt cagaagctta tcgataccgt cgactaaagc
     4141 caaatagaaa attattcagt tcctggctta agtttttaaa agtgatatta tttatttggt
     4201 tgtaaccaac caaaagaatg taaataacta atacataatt atgttagttt taagttagca
     4261 acaaattgat tttagctata ttagctactt ggttaataaa tagaatatat ttatttaaag
     4321 ataattgcgt ttttattgtc agggagtgag tttgcttaaa aactcgttta gatccactag
     4381 ttctagagcg gccgctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
     4441 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
     4501 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
     4561 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg
     4621 aggcgcgccc tagttccagt gaaatccaag cattttctaa attaaatgta ttcttattat
     4681 tatagttgtt atttttgata tatataaaca acactattat gcccaccatt tttttgagat
     4741 gcatctacac aaggaacaaa cactggatgt cactttcagt tcaaattgta acgctaatca
     4801 ctccgaacag gtcacaaaaa attaccttaa aaagtcataa tattaaatta gaataaatat
     4861 agctgtgagg gaaatatata caaatatatt ggagcaaata aattgtacat acaaatattt
     4921 attactaatt tctattgaga cgaaatgaac cactcggaac catttgagcg aaccgaatcg
     4981 cgcggaacta acgacagtcg ctccaaggtc gtcgaacaaa aggtgaatgt gttgcggaga
     5041 gcgggtggga gacagcgaaa gagcaactac gaaacgtggt gtggtggagg tgaattatga
     5101 agagggcgcg cgatttgaaa agtatgtata taaaaaatat atcccggtgt tttatgtagc
     5161 gataaacgag tttttgatgt aaggtatgca ggtgtgtaag tcttttggtt agaagacaaa
     5221 tccaaagtct acttgtgggg atgttcgaag gggaaatact tgtattctat aggtcatatc
     5281 ttgtttttat tggcacaaat ataattacat tagctttttg agggggcaat aaacagtaaa
     5341 cacgatggta ataatggtaa aaaaaaaaaa caagcagtta tttcggatat atgtcggcta
     5401 ctccttgcgt cgggcccgaa gtcttagagc cagatatgcg agcacccgga agctcacgat
     5461 gagaatggcc agacccacgt agtccagcgg cagatcggcg gcggagaagt taagcgtctc
     5521 caggatgacc ttgcccgaac tggggcacgt ggtgttcgac gatgtgcagc taatttcgcc
     5581 cggctccacg tccgcccatt ggttaatcag cagaccctcg ttggcgtaac ggaaccatga
     5641 gaggtacgac aaccatttga ggtatactgg caccgagccc gagttcaaga agaagccgcc
     5701 aaagagcagg aatggtatga taaccggcgg acccacagac agcgccatcg aggtcgagga
     5761 gctggcgcag gatattagat atccgaagga cgttgacaca ttggccacca gagtgaccag
     5821 cgccaggcag ttgaagaagt gcagcactcc ggcccgcagt ccgatcatcg gataggcaat
     5881 cgccgtgaag accagtggca ctgtgagaaa aagcggcaat tcggcaatcg ttttgcccag
     5941 aaagtatgtg tcacagcgat aaagtcgact tcgggcctcc ctcataaaaa ctggcagctc
     6001 tgaggtgaac acctaaatcg aatcgattca ttagaaagtt agtaaattat tgaaatgcaa
     6061 atgtattcta aacatgactt acatttatcg tggcaaagac gttttgaaag gtcatgttgg
     6121 tcaggaagag gaagatggct ccgttgatat tcatcacacc cacttgcgtg agttgttggc
     6181 ccaaaaagat gaggccaatc aagatggcaa ccatctgcaa attaaaatgt tactcgcatc
     6241 tcattaatat tcgcgagtta aatgaaattt atttatcttc tgcaaaacta taaactatac
     6301 atctcattga aaaaaactaa gaagggtgtg gaatcaggca attctatcta aaatctagcg
     6361 aatttgtttc caagaattgt aagcgttata tcatttgttt ccactggaac cactcaccgt
     6421 tgtctgaata agtcgcactt ttacgaggag tggttccttg agcaccgaca gccaggatcg
     6481 ccacaggacc gcccggaact gcatgaacca ggtggccttg taggtgtacc cattctccgg
     6541 ctgctccagt ggcttctcca gatttttggt ggccaacaac tgctccatat cccgggctac
     6601 tttgctaatg gcaaaattgt cgcatatctt ggcgatccga tcacgggact cgatctcccg
     6661 tccgggcaca acggccaaca cctgtacgta aaagtccgcc ggattgtagt tggtaggaca
     6721 ctgggcaccc acgctggata ggagttgaga tgttatgtaa tactagatac ccttaataaa
     6781 cacatcgaac tcactaggaa aagaagtcga cggcttcgct gggagtgccc aagaaagcta
     6841 ccctgccctc ggccatcaga aggatcttgt caaagagctc aaacagctcg gaagacggct
     6901 gatgaatggt caggatgacg gtcttgccct tctgcgacag cttcttcagc acctggacga
     6961 cgctgtgggc ggtaaaggag tccagtccgg aggtgggctc atcgcagatc agaagcggcg
     7021 gatcggttag agcctcggag gcgaatgcca gacgcttcct ttctccgccg gacagacctt
     7081 tcaccctgcc gggcacaccg atgatcgtgt gctgacattt gctgagcgaa agctcctgga
     7141 tcacctgatc cacgcgggcc actcgctgcc gataggtcag atgtcgtggc atccgcacca
     7201 tggcttggaa aatcaggtgt tccctggccg ttagggagcc gataaagagg tcatcctgct
     7261 ggacataggc gcacctggcc tgcatctcct tggcgtccac aggttggcca ttgagcagtc
     7321 gcatcccgga tggcgatact tggatgccct gcggcgatcg aaaggcaagg gcattcagca
     7381 gggtcgtctt tccggcaccg gaactgccca tcacggccaa aagttcgccc ggataggcca
     7441 cgccgcaaac tgagtttcaa attggtaatt ggacccttta ttaagatttc acacagatca
     7501 gccgactgcg aatagaaact caccgttctt gagcaaatgt ttcctgggcg ccggtatgtg
     7561 tcgctcgttg cagaatagtc cgcgtgtccg gttgaccagc tgccgccatc cggagcccgg
     7621 ctgattgacc gccccaaaga tgtccatatt gtgccaggca taggtgaggt tctcggctag
     7681 ttggccgctc cctgaaccgg agtcctccgg cggactgggt ggccggagcg tgccgtagtt
     7741 tttggcctgc ccgaagccct ggttaatgca gctctgcgaa gccgctccgc tgtcaccctg
     7801 caatgatagg ggatctcaaa tatcaactac tagcgttatg ctcatctaac cccgaacaaa
     7861 aagtaccccg aagtatccta cgaagtaggt ttatactttt atttattttt tgtgcatcta
     7921 ggatcagctt aaaatatctg gttgttatat tttttgtaaa aaagaatata gtcgaaaatg
     7981 aatgccttta gatgtcttga tcatgatatg atctcaaaaa ttgtcttata tagcgagaac
     8041 agctaccaga ataatctgtt tcgtgtcact atttgtttgt gcaattgcgg tttgggattt
     8101 ttgtgggtcg cagttctcac gccgcataca atttgatgtt gcaatcgcag ttcctataga
     8161 tcaagtgaac ttaagatgta tgcacatgta ctactcacat tgttcagatg ctcggcagat
     8221 gggtgtttgc tgcctccgcg aattaatagc tcctgatcct cttggcccat tgccgggatt
     8281 tttcacactt tcccctgctt acccacccaa aaccaatcac caccccaatc actcaaaaaa
     8341 caaacaaaaa taagaagcga gaggagtttt ggcacagcac tttgtgttta attgatggcg
     8401 taaaccgctt ggagcttcgt cacgaaaccg ctgacaaaat gcaactgaag gcggacattg
     8461 acgctacgta acgctacaaa cggtggcgaa agagatagcg gacgcagcgg cgaaagagac
     8521 ggcgatattt ctgtggacag agaaggaggc aaacagcgct gactttgagt ggaatgtcat
     8581 tttgagtgag aggtaatcga aagaacctgg tacatcaaat acccttggat cgaagtaaat
     8641 ttaaaactga tcagataagt tcaatgatat ccagtgcagt aaaaaaaaaa aatgtttttt
     8701 ttatctactt tccgcaaaaa tgggttttat taacttacat acatactagg cgcgcccata
     8761 tgttcggctt gtcgacatgc ccgccgtgac cgtcgagaac ccgctgacgc tgccccgcgt
     8821 atccgcaccc gccgacgccg tcgcacgtcc cgtgctcacc gtgaccaccg cgcccagcgg
     8881 tttcgagggc gagggcttcc cggtgcgccg cgcgttcgcc gggatcaact accgccacct
     8941 cgacccgttc atcatgatgg accagatggg tgaggtggag tacgcgcccg gggagcccaa
     9001 gggcacgccc tggcacccgc accgcggctt cgagaccgtg acctacatcg tcgacggtaa
     9061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9541 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10861 agtgccacct gacgtc
//
