LOCUS       sequence-158655-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23117_tetR PEZ1_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ05
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             45..56
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(65..99)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
ORIGIN
        1 gcgctgttaa tcactttact tttatctaat ctggacatct agtatttctc ctctttctct
       61 agtagctagc acaatcccta ggactgagct agctgtcaag gaattcttaa caaaatccct
      121 atcagtgata gaacaagatg taacatccct atcagtgata gagtataaag ttaactgtgg
      181 gattgcaaaa ggagctccca actcataaag tcaagtagga gattaatcat atgtcggtca
      241 tcaagcccga aatgaaaatt aaactgcgca tggagggcgc tgtgaacggg cacaagttcg
      301 tgatcgaagg tgaaggcatt ggtaagccgt atgaaggtac tcaaaccctg gacttaacgg
      361 ttaaagaagg tgccccgttg ccatttagct acgacatcct gacacccgct tttcagtatg
      421 gcaatcgcgc gttcaccaag tatccgaaag atattccgga ttatttcaag caggcattcc
      481 cggaaggata ctcatgggaa cgctcgatga cctacgaaga ccagggcatt tgcattgcca
      541 caagcgacat cacaatggag ggtgattgct ttttttacaa aattcgcttc gacgggacaa
      601 acttcccgcc gaatggccca gtgatgcaga aaaagacgct taaatgggaa ccgtcgacag
      661 agaaaatgta cgttcgcgat ggcgttctga aaggcgatgt taatatggcg ctgcttctcg
      721 aagggggagg ccattaccgt tgtgatttca aaaccacata caaagcaaaa aaagatgttc
      781 gtttgccgga cgcgcatgaa gtcgatcacc gcattgagat cctgtcacat gataaagatt
      841 ac
//
