LOCUS       sequence-158661-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for ORF expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pInducer20
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(171..788,789..794,795..830,831..833,834..869,870..872,
                     873..908,909..917)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
       61 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
      121 gtaccgagct cggatctcca ccccgtaccg gtcctgcagt cgaattcacc atgtctagac
      181 tggacaagag caaagtcata aacggagctc tggaattact caatggtgtc ggtatcgaag
      241 gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc
      301 acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc
      361 atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca
      421 agtcataccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc
      481 gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag
      541 gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct
      601 gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg
      661 attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag ggagccgaac
      721 ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg
      781 aaagcggcgg gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg
      841 cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg
      901 acatgctccc cgggtaacta agtaaggatc cgcggccgca cta
//
