LOCUS       sequence-158662-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  intronically embedded eSIBR backbone in gateway middle entry vector 
            (pDONR221).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Fowler DK, Williams C, Gerritsen AT, Washbourne P
  TITLE     Improved knockdown from artificial microRNAs in an enhanced miR-155 
            backbone: a designer's guide to potent multi-target RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Nov 17. pii: gkv1246.
  PUBMED    26582923
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 17. pii: gkv1246."
COMMENT     Sequence Label: pME-eSIBR
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 cttataatgc caactttgta caagaaagct gggtcagcac gttgcccagg agctgtagga
      121 aaaagaagaa ggcatgaaca tggttagcag aggggcccgg tttggactca gagtatttta
      181 tcctcatctc aaacagtgta tatcattgta accataaaga gaaaggcagg atgatgacca
      241 ggatgtagtt gtttctacca ataagaatat ttccacgcca gccagaattt atatgcagaa
      301 atattctacc ttatcattta attataacaa ttgttctcta aaactgtgct gaagtacaat
      361 ataatatacc ctgattgtct agaggctcga gttgtcatcc tcccacggtg gccatttgtt
      421 ccatgtgagt gctgataaag ggccttgtgt cctgtgtctt ccagatcttg aagacaacag
      481 catacagccc aaagcaagcc tccagtcgac tttgaattcg aatcgatggg atcttacaat
      541 ctgacaaata aacaaaagtg aatttaaaaa ttcgttacaa atgcaagcta aagtttaacg
      601 aaaaagttac agaaaatgaa aagaaaataa gaggagacaa tggttgtcaa cagagtagaa
      661 agtgaaagaa acaaaattat catgagggtc catgcatggt gatacaaggg acatcttccc
      721 attctaaaca acaccctgaa aactttgccc cctccatata acatgaattt tacaatagcg
      781 aaaaagaaag aacaatcaag ggtccccaaa ctcaccctga agttctcagg cgcgccttag
      841 cttcgtctaa caaaaaagcc aaaaacggcc agaatttagc ggacaattta ctagtctaac
      901 actgaaaatt acatattgac ccaaatgatt acatttcaaa aggtgc
//
