LOCUS       sequence-158663-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  intronically embedded eSIBR backbone in gateway middle entry vector 
            (pDONR221).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Fowler DK, Williams C, Gerritsen AT, Washbourne P
  TITLE     Improved knockdown from artificial microRNAs in an enhanced miR-155 
            backbone: a designer's guide to potent multi-target RNAi.
  JOURNAL   Nucleic Acids Res. 2015 Nov 17. pii: gkv1246.
  PUBMED    26582923
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 17. pii: gkv1246."
COMMENT     Sequence Label: pME-eSIBR
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..31
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15..31
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    47..146
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     740..759
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 ccagtcacga cgttgtaaaa cgacggccag tcttaagctc gggccccaaa taatgatttt
       61 attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct tttttataat
      121 gccaactttg tacaaaaaag caggctggat cgctgtgatc gtcacttggt gagtagcggg
      181 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
      241 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
      301 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatagaacct tcgctaatgc
      361 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
      421 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
      481 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
      541 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
      601 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
      661 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
      721 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
      781 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
      841 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
      901 gacgaa
//
