LOCUS       sequence-158670-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active NFAT1, mutated to interfere selectively with 
            the NFAT:AP-1 interactio. Contains Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, 
            Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, 
            Mempel TR, Lahdesmaki H, Hogan PG, Rao A
  TITLE     The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T
            Cells.
  JOURNAL   Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006.
  PUBMED    25680272
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006."
COMMENT     Sequence Label: CA-RIT-NFAT1 (PGK-Puro)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(185..204)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(549..568)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     668..690
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(728..746)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aataataaat cttttatttt atctatggnt cgtactctat agcgtcttcn tcgtggctga
       61 tattgttgag tcaanactag agcctggacc actgatatcn tgtctttaac aaattggact
      121 aatcncctct acgncggacn catcgtggcc ggcatcanng gcgccacagg tgcggttgct
      181 ggcncctata tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg
      241 ancgcttgtn tcngcntggg tatggtggca ggccncgtgn ccnggggact gttgacgcgc
      301 catctccttg catgcaccat tccttgcgcc gtcgttttac aacgtcntga ctggnaaaac
      361 cctggcgtta cccaacttaa tcgncttgca gcacatcccc ctttcgccag ctggcgtaat
      421 aangaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      481 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
      541 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
      601 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
      661 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
      721 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      781 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      841 taaatacatt caaatatgta tccgctcatg agacaataac ccctgataaa tgcttcaata
      901 atattgaaaa aagnaanant atgagtantt caacatttcc gtgtcc
//
