LOCUS       sequence-158671-       11386 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Gateway cloning of promoter elements upstream of a 
            GFP::Luciferase(nls) reporter, facilitating qualitative and 
            quantitative analysis of expression in transgenic fly lines and S2 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11386)
  AUTHORS   Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, 
            Harrison MM, Lee CY
  TITLE     An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and 
            Rapid Restriction of Developmental Potential during Asymmetric Stem 
            Cell Division.
  JOURNAL   Dev Cell. 2017 Feb 27;40(4):367-380.e7. doi: 
            10.1016/j.devcel.2017.01.014.
  PUBMED    28245922
REFERENCE   2  (bases 1 to 11386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.01"; journalName: "Dev Cell"; date: 
            "2017-02-27- 27"; volume: "40"; issue: "4"; pages: "367-380.e7"
COMMENT     Sequence Label: VanGlow-GL
FEATURES             Location/Qualifiers
     source          1..11386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(1903..1905,1906..1908,1909..2619)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4882..4898)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4882..4898)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5909..6523,6594..6725,6929..7244,7306..7960,
                     8035..8308,8710..8781))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     9233..9251
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    9476..9545
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(9632..10220)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9712..9731)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10391..11182,11183..11251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11014..11033
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11252..11356)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccagatctgg taccagatcc gggatcccgt aagcaaataa acaagcgcag ctgaacaagc
     1861 taaacaatct gcagcccaag cttgaagcaa gcctcctgca acatggtgtc caagggcgag
     1921 gagctgttca ccggcgtggt gccaattctg gtggagctgg atggcgacgt gaacggccac
     1981 aagttcagcg tgtccggcga gggcgagggc gacgccacct atggaaagct gaccctgaag
     2041 ttcatctgca ccaccggcaa gctgcccgtg ccatggccaa ccctcgtgac caccctgacc
     2101 tatggcgtgc agtgcttcag ccgctacccc gatcacatga agcagcacga tttcttcaag
     2161 agcgccatgc ccgagggcta cgtgcaggag cgcaccatct ttttcaagga tgacggcaac
     2221 tacaagaccc gcgccgaagt gaagttcgag ggcgataccc tcgtgaaccg catcgagctg
     2281 aagggcatcg atttcaagga ggatggaaac atcctgggcc acaagctgga gtacaactac
     2341 aacagccaca acgtgtacat catggccgac aagcagaaga acggcatcaa agtgaacttc
     2401 aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgatcacta ccagcagaac
     2461 accccaatcg gcgacggccc agtgctgctg cccgataacc attacctgag cacccagagc
     2521 gccctgagca aggatcccaa cgagaagcgc gaccacatgg tgctgctgga gtttgtgacc
     2581 gccgccggca ttaccctggg catggatgag ctgtacaaga agaacatcaa gaagggccca
     2641 gccccgttct accccctgga ggacggaacc gccggagagc agctgcacaa ggccatgaag
     2701 cgctacgccc tggtgcccgg cacgatcgcc tttaccgatg cccacatcga ggtggacatc
     2761 acctacgccg agtacttcga gatgagcgtg cgcctggccg aggccatgaa gcgctatggc
     2821 ctgaacacca accaccgcat cgtcgtgtgc agcgagaaca gcctgcagtt cttcatgccc
     2881 gtgctgggcg ccctgttcat tggagtggcc gtggccccag ccaacgatat ctacaacgag
     2941 cgcgagctgc tgaacagcat gggcatcagc cagcccaccg tggtgttcgt gtccaagaag
     3001 ggactgcaga agatcctgaa cgtgcagaag aagctgccca tcatccagaa gatcatcatc
     3061 atggacagca agaccgacta ccagggcttc cagagcatgt acaccttcgt gaccagccat
     3121 ctgccacccg gcttcaacga gtacgatttc gtgcccgaga gcttcgatcg cgataagacc
     3181 attgccctga tcatgaacag cagcggcagc accggactgc caaagggcgt ggccctgcca
     3241 caccgcaccg cctgcgtgcg ctttagtcat gcccgcgatc ccatcttcgg caaccagatc
     3301 atccccgata ccgccatcct gtccgtggtg cccttccacc acggcttcgg catgttcacc
     3361 accctgggct acctgatctg cggcttccgc gtggtgctga tgtaccgctt cgaggaggag
     3421 ctgtttctgc gcagcctgca ggattacaag atccagtccg ccctgctggt gcccaccctg
     3481 ttctcgttct tcgccaagag caccctgatc gataagtacg atctgagcaa cctgcacgag
     3541 atcgccagcg gcggagcccc actgtccaag gaagtgggcg aggccgtggc caagcgcttt
     3601 catctgcccg gaattcgcca gggctacggc ctgaccgaga ccaccagcgc cattctgatc
     3661 accccagagg gcgacgataa gccaggcgcc gtgggaaagg tggtgccgtt tttcgaggcc
     3721 aaggtggtgg atctggacac gggcaagacc ctgggagtga atcagcgcgg agagctgtgc
     3781 gtgcgcggac cgatgattat gagcggctac gtgaacaacc ccgaggccac caacgccctg
     3841 attgacaagg atggctggct gcacagcggc gatattgcct actgggatga ggatgagcac
     3901 ttcttcatcg tggatcgcct gaagtccctg atcaagtaca agggctacca ggtggcccca
     3961 gccgagctgg agagtatcct gctgcagcac cccaacatct tcgatgccgg cgtggccggc
     4021 ctgcccgatg atgacgccgg cgagctgcca gccgccgtgg tggtgctgga gcatggaaag
     4081 accatgaccg agaaggagat cgtggattac gtggccagcc aagtgaccac cgccaagaag
     4141 ctgcgcggag gcgtggtgtt tgtggatgag gtgcccaagg gcctgacggg caagctggat
     4201 gcccgcaaga ttcgcgagat cctgattaag gccaagaagg gcggcaagat cgccgtgtcc
     4261 cgccaccgcc gccatcgcca gcgcagtcgc agccgaaatc gcagtcgatc ccgaagtagc
     4321 gagcgaaagc gacgacagcg ctcccgctcg cgcagttccg agcgtcgacg ctacctgtac
     4381 aagtagaatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg
     4441 caccatctca atttctttca tttatacatc gttttgcctt cttttatgta actatactcc
     4501 tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa atgactagaa
     4561 ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac gagggcttat
     4621 tcagaagctt atcgataccg tcgactaaag ccaaatagaa aattattcag ttcctggctt
     4681 aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat gtaaataact
     4741 aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat attagctact
     4801 tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt cagggagtga
     4861 gtttgcttaa aaactcgttt agatccacta gttctagagc ggccgctggc cacgggtgcg
     4921 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     4981 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     5041 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     5101 gaagtcagcg ccctgcacca ttatgttccg gaggcgcgcc ctagttccag tgaaatccaa
     5161 gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat atatataaac
     5221 aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa acactggatg
     5281 tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa aattacctta
     5341 aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat acaaatatat
     5401 tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag acgaaatgaa
     5461 ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc gctccaaggt
     5521 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
     5581 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
     5641 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
     5701 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
     5761 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
     5821 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
     5881 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     5941 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagacccacg tagtccagcg
     6001 gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg
     6061 tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca
     6121 gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg
     6181 gcaccgagcc cgagttcaag aagaagccgc caaagagcag gaatggtatg ataaccggcg
     6241 gacccacaga cagcgccatc gaggtcgagg agctggcgca ggatattaga tatccgaagg
     6301 acgttgacac attggccacc agagtgacca gcgccaggca gttgaagaag tgcagcactc
     6361 cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa
     6421 aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt gtcacagcga taaagtcgac
     6481 ttcgggcctc cctcataaaa actggcagct ctgaggtgaa cacctaaatc gaatcgattc
     6541 attagaaagt tagtaaatta ttgaaatgca aatgtattct aaacatgact tacatttatc
     6601 gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc tccgttgata
     6661 ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat caagatggca
     6721 accatctgca aattaaaatg ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt
     6781 tatttatctt ctgcaaaact ataaactata catctcattg aaaaaaacta agaagggtgt
     6841 ggaatcaggc aattctatct aaaatctagc gaatttgttt ccaagaattg taagcgttat
     6901 atcatttgtt tccactggaa ccactcaccg ttgtctgaat aagtcgcact tttacgagga
     6961 gtggttcctt gagcaccgac agccaggatc gccacaggac cgcccggaac tgcatgaacc
     7021 aggtggcctt gtaggtgtac ccattctccg gctgctccag tggcttctcc agatttttgg
     7081 tggccaacaa ctgctccata tcccgggcta ctttgctaat ggcaaaattg tcgcatatct
     7141 tggcgatccg atcacgggac tcgatctccc gtccgggcac aacggccaac acctgtacgt
     7201 aaaagtccgc cggattgtag ttggtaggac actgggcacc cacgctggat aggagttgag
     7261 atgttatgta atactagata cccttaataa acacatcgaa ctcactagga aaagaagtcg
     7321 acggcttcgc tgggagtgcc caagaaagct accctgccct cggccatcag aaggatcttg
     7381 tcaaagagct caaacagctc ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc
     7441 ttctgcgaca gcttcttcag cacctggacg acgctgtggg cggtaaagga gtccagtccg
     7501 gaggtgggct catcgcagat cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc
     7561 agacgcttcc tttctccgcc ggacagacct ttcaccctgc cgggcacacc gatgatcgtg
     7621 tgctgacatt tgctgagcga aagctcctgg atcacctgat ccacgcgggc cactcgctgc
     7681 cgataggtca gatgtcgtgg catccgcacc atggcttgga aaatcaggtg ttccctggcc
     7741 gttagggagc cgataaagag gtcatcctgc tggacatagg cgcacctggc ctgcatctcc
     7801 ttggcgtcca caggttggcc attgagcagt cgcatcccgg atggcgatac ttggatgccc
     7861 tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct ttccggcacc ggaactgccc
     7921 atcacggcca aaagttcgcc cggataggcc acgccgcaaa ctgagtttca aattggtaat
     7981 tggacccttt attaagattt cacacagatc agccgactgc gaatagaaac tcaccgttct
     8041 tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc
     8101 ggttgaccag ctgccgccat ccggagcccg gctgattgac cgccccaaag atgtccatat
     8161 tgtgccaggc ataggtgagg ttctcggcta gttggccgct ccctgaaccg gagtcctccg
     8221 gcggactggg tggccggagc gtgccgtagt ttttggcctg cccgaagccc tggttaatgc
     8281 agctctgcga agccgctccg ctgtcaccct gcaatgatag gggatctcaa atatcaacta
     8341 ctagcgttat gctcatctaa ccccgaacaa aaagtacccc gaagtatcct acgaagtagg
     8401 tttatacttt tatttatttt ttgtgcatct aggatcagct taaaatatct ggttgttata
     8461 ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt agatgtcttg atcatgatat
     8521 gatctcaaaa attgtcttat atagcgagaa cagctaccag aataatctgt ttcgtgtcac
     8581 tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc gcagttctca cgccgcatac
     8641 aatttgatgt tgcaatcgca gttcctatag atcaagtgaa cttaagatgt atgcacatgt
     8701 actactcaca ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc gaattaatag
     8761 ctcctgatcc tcttggccca ttgccgggat ttttcacact ttcccctgct tacccaccca
     8821 aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt
     8881 tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc
     8941 gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa acggtggcga
     9001 aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg
     9061 caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg
     9121 gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata
     9181 tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa atgggtttta
     9241 ttaacttaca tacatactag gcgcgcccat atgttcggct tgtcgacatg cccgccgtga
     9301 ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc
     9361 ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc
     9421 gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg gaccagatgg
     9481 gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg caccgcggct
     9541 tcgagaccgt gacctacatc gtcgacggta acatgtgagc aaaaggccag caaaaggcca
     9601 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9661 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9721 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9781 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9841 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9901 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9961 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10021 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    10081 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10141 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10201 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10261 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10321 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10381 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10441 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10501 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10561 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10621 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10681 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10741 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10801 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10861 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10921 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10981 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11041 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11101 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11161 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11221 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11281 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11341 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgagtc
//
