LOCUS       sequence-158672-       10726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for Gateway cloning of promoter elements upstream of a 
            mCherry::Renilla(nls) reporter, facilitating qualitative and 
            quantitative analysis of expression in transgenic fly lines and S2 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Janssens DH, Hamm DC, Anhezini L, Xiao Q, Siller KH, Siegrist SE, 
            Harrison MM, Lee CY
  TITLE     An Hdac1/Rpd3-Poised Circuit Balances Continual Self-Renewal and 
            Rapid Restriction of Developmental Potential during Asymmetric Stem 
            Cell Division.
  JOURNAL   Dev Cell. 2017 Feb 27;40(4):367-380.e7. doi: 
            10.1016/j.devcel.2017.01.014.
  PUBMED    28245922
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.01"; journalName: "Dev Cell"; date: 
            "2017-02-27- 27"; volume: "40"; issue: "4"; pages: "367-380.e7"
COMMENT     Sequence Label: VanGlow-RR
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4222..4238)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4222..4238)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(join(5249..5863,5934..6065,6269..6584,6646..7300,
                     7375..7648,8050..8121))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8573..8591
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8816..8885
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8972..9560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9052..9071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9731..10522,10523..10591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10354..10373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10592..10696)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccagatctgg taccagatcc gggatcccgt aagcaaataa acaagcgcag ctgaacaagc
     1861 taaacaatct gcagcccaag cttgaagcaa gcctcctgca acatggtgtc caagggcgag
     1921 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggcagc
     1981 gtgaacggcc acgagttcga gattgagggc gagggcgagg gacgcccata tgagggaacc
     2041 cagaccgcca agctgaaagt gaccaagggc ggacccctgc ccttcgcctg ggatattctg
     2101 agcccccagt ttatgtacgg cagcaaggcc tacgtgaagc accccgccga tatccccgat
     2161 tacctgaagc tgagcttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggat
     2221 ggcggcgtcg tgaccgtgac ccaggatagc agtctgcagg atggcgagtt catctacaaa
     2281 gtgaagctgc gcggcaccaa cttccccagc gacggaccag tgatgcagaa gaagaccatg
     2341 ggctgggagg ccagcagcga gcgcatgtat ccagaggacg gcgccctgaa gggcgagatc
     2401 aagcagcgcc tgaagctgaa ggatggcggc cactacgatg ccgaagtgaa gaccacctac
     2461 aaggccaaga agcccgtgca gctgccaggc gcctacaacg tgaacatcaa gctggatatc
     2521 accagccaca acgaggacta caccatcgtg gagcagtacg agcgcgccga gggccgccat
     2581 agtaccggcg gaatggatga gctgtacaag ggcaccagcg gcagcggaag tggaagcgga
     2641 tccggcagtg gatccggaag tgcctgcacc agcaaggtgt acgatcccga gcagcgcaag
     2701 cgcatgatca ccggaccaca gtggtgggcc cgctgcaagc agatgaacgt gctggatagc
     2761 ttcatcaact actacgacag cgagaagcac gccgagaacg ccgtgatctt cctgcacgga
     2821 aatgccgcca gcagctacct gtggcgccat gtggtgccac acatcgagcc agtggcccgc
     2881 tgcatcatcc ccgatctgat cggcatgggc aagagcggca agtccggcaa tggaagctac
     2941 cgcctgctgg atcactacaa gtacctgacc gcctggttcg agctgctgaa cctgcccaag
     3001 aagatcattt tcgtgggcca cgattggggc gcctgcctgg ccttccatta cagctacgag
     3061 caccaggata agatcaaggc catcgtgcat gccgagagcg tggtggatgt gatcgagagc
     3121 tgggatgagt ggcccgatat cgaggaggac attgccctga tcaagtccga ggagggcgag
     3181 aagatggtgc tggagaacaa cttcttcgtg gagaccatgc tgccgagcaa gattatgcgc
     3241 aagctggagc ccgaggagtt cgccgcctat ctggagccct tcaaggagaa gggcgaagtg
     3301 cgccgcccaa ccctgagttg gccacgcgag attccactcg tgaagggcgg caagcccgat
     3361 gtggtgcaga tcgtgcgcaa ctacaacgcc tacctgcgcg cctccgatga tctgccgaag
     3421 atgttcatcg agagcgatcc cggcttcttc agcaacgcca ttgtggaggg cgccaagaag
     3481 ttccccaaca ccgagttcgt gaaagtgaag ggcctgcact tcagccagga ggatgcccca
     3541 gacgagatgg gcaagtacat caagtcgttc gtggagcgcg tgctgaagaa cgagcagagt
     3601 cgccatcgcc gccaccgcca gcgcagtcgc agccgcaatc gcagtcgctc ccgcagtagc
     3661 gagcgcaagc gtcgacagcg ctcccgcagc cgaagttccg agcgtcgacg ctacctgtac
     3721 aagtagaatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact gtgcatcgtg
     3781 caccatctca atttctttca tttatacatc gttttgcctt cttttatgta actatactcc
     3841 tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa atgactagaa
     3901 ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac gagggcttat
     3961 tcagaagctt atcgataccg tcgactaaag ccaaatagaa aattattcag ttcctggctt
     4021 aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat gtaaataact
     4081 aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat attagctact
     4141 tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt cagggagtga
     4201 gtttgcttaa aaactcgttt agatccacta gttctagagc ggccgctggc cacgggtgcg
     4261 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     4321 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     4381 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     4441 gaagtcagcg ccctgcacca ttatgttccg gaggcgcgcc ctagttccag tgaaatccaa
     4501 gcattttcta aattaaatgt attcttatta ttatagttgt tatttttgat atatataaac
     4561 aacactatta tgcccaccat ttttttgaga tgcatctaca caaggaacaa acactggatg
     4621 tcactttcag ttcaaattgt aacgctaatc actccgaaca ggtcacaaaa aattacctta
     4681 aaaagtcata atattaaatt agaataaata tagctgtgag ggaaatatat acaaatatat
     4741 tggagcaaat aaattgtaca tacaaatatt tattactaat ttctattgag acgaaatgaa
     4801 ccactcggaa ccatttgagc gaaccgaatc gcgcggaact aacgacagtc gctccaaggt
     4861 cgtcgaacaa aaggtgaatg tgttgcggag agcgggtggg agacagcgaa agagcaacta
     4921 cgaaacgtgg tgtggtggag gtgaattatg aagagggcgc gcgatttgaa aagtatgtat
     4981 ataaaaaata tatcccggtg ttttatgtag cgataaacga gtttttgatg taaggtatgc
     5041 aggtgtgtaa gtcttttggt tagaagacaa atccaaagtc tacttgtggg gatgttcgaa
     5101 ggggaaatac ttgtattcta taggtcatat cttgttttta ttggcacaaa tataattaca
     5161 ttagcttttt gagggggcaa taaacagtaa acacgatggt aataatggta aaaaaaaaaa
     5221 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     5281 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagacccacg tagtccagcg
     5341 gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa ctggggcacg
     5401 tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat tggttaatca
     5461 gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg aggtatactg
     5521 gcaccgagcc cgagttcaag aagaagccgc caaagagcag gaatggtatg ataaccggcg
     5581 gacccacaga cagcgccatc gaggtcgagg agctggcgca ggatattaga tatccgaagg
     5641 acgttgacac attggccacc agagtgacca gcgccaggca gttgaagaag tgcagcactc
     5701 cggcccgcag tccgatcatc ggataggcaa tcgccgtgaa gaccagtggc actgtgagaa
     5761 aaagcggcaa ttcggcaatc gttttgccca gaaagtatgt gtcacagcga taaagtcgac
     5821 ttcgggcctc cctcataaaa actggcagct ctgaggtgaa cacctaaatc gaatcgattc
     5881 attagaaagt tagtaaatta ttgaaatgca aatgtattct aaacatgact tacatttatc
     5941 gtggcaaaga cgttttgaaa ggtcatgttg gtcaggaaga ggaagatggc tccgttgata
     6001 ttcatcacac ccacttgcgt gagttgttgg cccaaaaaga tgaggccaat caagatggca
     6061 accatctgca aattaaaatg ttactcgcat ctcattaata ttcgcgagtt aaatgaaatt
     6121 tatttatctt ctgcaaaact ataaactata catctcattg aaaaaaacta agaagggtgt
     6181 ggaatcaggc aattctatct aaaatctagc gaatttgttt ccaagaattg taagcgttat
     6241 atcatttgtt tccactggaa ccactcaccg ttgtctgaat aagtcgcact tttacgagga
     6301 gtggttcctt gagcaccgac agccaggatc gccacaggac cgcccggaac tgcatgaacc
     6361 aggtggcctt gtaggtgtac ccattctccg gctgctccag tggcttctcc agatttttgg
     6421 tggccaacaa ctgctccata tcccgggcta ctttgctaat ggcaaaattg tcgcatatct
     6481 tggcgatccg atcacgggac tcgatctccc gtccgggcac aacggccaac acctgtacgt
     6541 aaaagtccgc cggattgtag ttggtaggac actgggcacc cacgctggat aggagttgag
     6601 atgttatgta atactagata cccttaataa acacatcgaa ctcactagga aaagaagtcg
     6661 acggcttcgc tgggagtgcc caagaaagct accctgccct cggccatcag aaggatcttg
     6721 tcaaagagct caaacagctc ggaagacggc tgatgaatgg tcaggatgac ggtcttgccc
     6781 ttctgcgaca gcttcttcag cacctggacg acgctgtggg cggtaaagga gtccagtccg
     6841 gaggtgggct catcgcagat cagaagcggc ggatcggtta gagcctcgga ggcgaatgcc
     6901 agacgcttcc tttctccgcc ggacagacct ttcaccctgc cgggcacacc gatgatcgtg
     6961 tgctgacatt tgctgagcga aagctcctgg atcacctgat ccacgcgggc cactcgctgc
     7021 cgataggtca gatgtcgtgg catccgcacc atggcttgga aaatcaggtg ttccctggcc
     7081 gttagggagc cgataaagag gtcatcctgc tggacatagg cgcacctggc ctgcatctcc
     7141 ttggcgtcca caggttggcc attgagcagt cgcatcccgg atggcgatac ttggatgccc
     7201 tgcggcgatc gaaaggcaag ggcattcagc agggtcgtct ttccggcacc ggaactgccc
     7261 atcacggcca aaagttcgcc cggataggcc acgccgcaaa ctgagtttca aattggtaat
     7321 tggacccttt attaagattt cacacagatc agccgactgc gaatagaaac tcaccgttct
     7381 tgagcaaatg tttcctgggc gccggtatgt gtcgctcgtt gcagaatagt ccgcgtgtcc
     7441 ggttgaccag ctgccgccat ccggagcccg gctgattgac cgccccaaag atgtccatat
     7501 tgtgccaggc ataggtgagg ttctcggcta gttggccgct ccctgaaccg gagtcctccg
     7561 gcggactggg tggccggagc gtgccgtagt ttttggcctg cccgaagccc tggttaatgc
     7621 agctctgcga agccgctccg ctgtcaccct gcaatgatag gggatctcaa atatcaacta
     7681 ctagcgttat gctcatctaa ccccgaacaa aaagtacccc gaagtatcct acgaagtagg
     7741 tttatacttt tatttatttt ttgtgcatct aggatcagct taaaatatct ggttgttata
     7801 ttttttgtaa aaaagaatat agtcgaaaat gaatgccttt agatgtcttg atcatgatat
     7861 gatctcaaaa attgtcttat atagcgagaa cagctaccag aataatctgt ttcgtgtcac
     7921 tatttgtttg tgcaattgcg gtttgggatt tttgtgggtc gcagttctca cgccgcatac
     7981 aatttgatgt tgcaatcgca gttcctatag atcaagtgaa cttaagatgt atgcacatgt
     8041 actactcaca ttgttcagat gctcggcaga tgggtgtttg ctgcctccgc gaattaatag
     8101 ctcctgatcc tcttggccca ttgccgggat ttttcacact ttcccctgct tacccaccca
     8161 aaaccaatca ccaccccaat cactcaaaaa acaaacaaaa ataagaagcg agaggagttt
     8221 tggcacagca ctttgtgttt aattgatggc gtaaaccgct tggagcttcg tcacgaaacc
     8281 gctgacaaaa tgcaactgaa ggcggacatt gacgctacgt aacgctacaa acggtggcga
     8341 aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca gagaaggagg
     8401 caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg aaagaacctg
     8461 gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag ttcaatgata
     8521 tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa atgggtttta
     8581 ttaacttaca tacatactag gcgcgcccat atgttcggct tgtcgacatg cccgccgtga
     8641 ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc gtcgcacgtc
     8701 ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc ccggtgcgcc
     8761 gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg gaccagatgg
     8821 gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg caccgcggct
     8881 tcgagaccgt gacctacatc gtcgacggta acatgtgagc aaaaggccag caaaaggcca
     8941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9421 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgagtc
//
