LOCUS       sequence-158678-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23113_tetR PEZ2_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ08
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             57..68
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(77..111)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 agcagctcta atgcgctgtt aatcacttta cttttatcta atctggacat ctagtatttc
       61 tcctctttct ctagtagcta gcataatccc taggactgag ctagccatca ggttaacaaa
      121 aaagcaggaa tatccctatc agtgatagac agacatccct atcagtgata gatataaagt
      181 taactgtggg attgcaaaag gagctcccaa ctcataaagt caagtaggag attaatcata
      241 tgtcggtcat caagcccgaa atgaaaatta aactgcgcat ggagggcgct gtgaacgggc
      301 acaagttcgt gatcgaaggt gaaggcattg gtaagccgta tgaaggtact caaaccctgg
      361 acttaacggt taaagaaggt gccccgttgc catttagcta cgacatcctg acacccgctt
      421 ttcagtatgg caatcgcgcg ttcaccaagt atccgaaaga tattccggat tatttcaagc
      481 aggcattccc ggaaggatac tcatgggaac gctcgatgac ctacgaagac cagggcattt
      541 gcattgccac aagcgacatc acaatggagg gtgattgctt tttttacaaa attcgcttcg
      601 acgggacaaa cttcccgccg aatggcccag tgatgcagaa aaagacgctt aaatgggaac
      661 cgtcgacaga gaaaatgtac gttcgcgatg gcgttctgaa aggcgatgtt aatatggcgc
      721 tgcttctcga agggggaggc cattaccgtt gtgatttcaa a
//
