LOCUS       sequence-158686-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 
            binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-SMVP-Cas9N-U6-gRNA P2
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..52
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccgctagcg ctaccggtcg ccaccatggc cccaaagaag aagcggaagg tcggtatcca
       61 cggagtccca gcagccgaca agaagtactc cattgggctc gatatcggca caaacagcgt
      121 cggctgggcc gtcattacgg acgagtacaa ggtgccgagc aaaaaattca aagttctggg
      181 caataccgat cgccacagca taaagaagaa cctcattggc gccctcctgt tcgactccgg
      241 ggaaacggcc gaagccacgc ggctcaaaag aacagcacgg cgcagatata cccgcagaaa
      301 gaatcggatc tgctacctgc aggagatctt tagtaatgag atggctaagg tggatgactc
      361 tttcttccat aggctggagg agtccttttt ggtggaggag gataaaaagc acgagcgcca
      421 cccaatcttt ggcaatatcg tggacgaggt ggcgtaccat gaaaagtacc caaccatata
      481 tcatctgagg aagaagcttg tagacagtac tgataaggct gacttgcggt tgatctatct
      541 cgcgctggcg catatgatca aatttcgggg acacttcctc atcgaggggg acctgaaccc
      601 agacaacagc gatgtcgaca aactctttat ccaactggtt cagacttaca atcagctttt
      661 cgaagagaac ccgatcaacg catccggagt tgacgccaaa gcaatcctga gcgctaggct
      721 gtccaaatcc cggcggctcg aaaacctcat cgcacagctc cctggggaga agaagaacgg
      781 cctgtttggt aatcttatcg ccctgtcact cgggctgacc cccaacttta a
//
