LOCUS       sequence-158693-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 
            binding..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-SMVP-Cas9N-U6-gRNA P1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..53
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agccgctagc gctaccggtc gccaccatgg ccccaaagaa gaagcggaag gtcggtatcc
       61 acggagtccc agcagccgac aagaagtact ccattgggct cgatatcggc acaaacagcg
      121 tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc aaagttctgg
      181 gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg ttcgactccg
      241 gggaaacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat acccgcagaa
      301 agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag gtggatgact
      361 ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag cacgagcgcc
      421 acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac ccaaccatat
      481 atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg ttgatctatc
      541 tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg gacctgaacc
      601 cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac aatcagcttt
      661 tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg agcgctaggc
      721 tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag aagaagaacg
      781 gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt aaatctaact
      841 tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat gatgatctcg
//
