LOCUS       sequence-158697-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C in mammalian cells; co-expresses turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CASI-Cas9C-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(97..108)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      705..714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      709..718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag ctgccgccaa taatttttca
       61 ttttcttgac aacttcttct gaggggacgt tatcactctt ccctctattt ttatcggatc
      121 ttgtcaacac tttattatca atagaatcat ctttgagaaa agactggggc acgatatgat
      181 ccacgtcgta gtcggagagc cgattgatgt ccagttcctg atccacgtac atgtccctgc
      241 cgttctgcag gtagtacagg tagagcttct cattctgaag ctgggtgttt tcaactgggt
      301 gttccttaag gatttgggac cccagttctt ttataccctc ttcaatcctc ttcatccttt
      361 ccctactgtt cttctgtccc ttctgggtag tttggttctc tcgggccatc tcgataacga
      421 tattctcggg cttatgcctt cccattactt tgacgagttc atccacgacc ttaacggtct
      481 gcagtattcc ctttttgata gctgggctac ctgcaagatt agcgatgtgc tcgtgaagac
      541 tgtccccctg gccagagtta tgcgcaataa tatcgttcgc cacaaagtta tgcgggcccg
      601 gcacggtcag atcaaacact tcttccacgc catccggttc aatgctcaca atcggatccc
      661 aatacacatc gctctgcgcc agctgacgca gttccgggca cgccgccgcc atggtggcgg
      721 cgctagcctg ttcgtcaccc aggacctgta gaaaaaaaaa gaaaacatga acatggttag
      781 tagaggcatc atcggcgttc accgccccac ggagatccct ccgcagaatc gccgagaagg
      841 gactactt
//
