LOCUS       sequence-158698-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cas9C in mammalian cells; co-expresses turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pAAV-CASI-Cas9C-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             137..193
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 ggaggtcctg gacgccacac tgattcatca gtcaattacg gggctctatg aaacaagaat
       61 cgacctctct cagctcggtg gagacagcag ggctgacccc aagaagaaga ggaaggtgtc
      121 tcgagctgga tccggagcca cgaacttctc tctgttaaag caagcagggg acgtggaaga
      181 aaaccccggt cctatgcccg ccatgaagat cgagtgccgc atcaccggca ccctgaacgg
      241 cgtggagttc gagctggtgg gcggcggaga gggcaccccc gagcagggcc gcatgaccaa
      301 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
      361 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
      421 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
      481 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
      541 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
      601 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
      661 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
      721 caagagcgcc atccacc
//
