LOCUS       sequence-158701-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that expresses Cas9FL in mammalian cells; co-expresses 
            turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pSMVP-Cas9FL-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agccgctagc gctaccggtc gccaccatgg acaagaagta ctccattggg ctcgatatcg
       61 gcacaaacag cgtcggctgg gccgtcatta cggacgagta caaggtgccg agcaaaaaat
      121 tcaaagttct gggcaatacc gatcgccaca gcataaagaa gaacctcatt ggcgccctcc
      181 tgttcgactc cggggaaacg gccgaagcca cgcggctcaa aagaacagca cggcgcagat
      241 atacccgcag aaagaatcgg atctgctacc tgcaggagat ctttagtaat gagatggcta
      301 aggtggatga ctctttcttc cataggctgg aggagtcctt tttggtggag gaggataaaa
      361 agcacgagcg ccacccaatc tttggcaata tcgtggacga ggtggcgtac catgaaaagt
      421 acccaaccat atatcatctg aggaagaagc ttgtagacag tactgataag gctgacttgc
      481 ggttgatcta tctcgcgctg gcgcatatga tcaaatttcg gggacacttc ctcatcgagg
      541 gggacctgaa cccagacaac agcgatgtcg acaaactctt tatccaactg gttcagactt
      601 acaatcagct tttcgaagag aacccgatca acgcatccgg agttgacgcc aaagcaatcc
      661 tgagcgctag gctgtccaaa tcccggcggc tcgaaaacct catcgcacag ctccctgggg
      721 agaagaagaa cggcctgttt ggtaatctta tcgccctgtc actcgggctg acccccaact
      781 ttaaatctaa cttcgac
//
