LOCUS       sequence-158702-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that expresses Cas9FL in mammalian cells; co-expresses 
            turboGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Chew WL, Tabebordbar M, Cheng JK, Mali P, Wu EY, Ng AH, Zhu K, 
            Wagers AJ, Church GM
  TITLE     A multifunctional AAV-CRISPR-Cas9 and its host response.
  JOURNAL   Nat Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993.
  PUBMED    27595405
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Sep 5. doi: 10.1038/nmeth.3993."
COMMENT     Sequence Label: pSMVP-Cas9FL-P2A-turboGFP
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(643..699)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(709..729)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(733..753)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(757..777)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggcgtgctgg tactccacga tgcccagctc ggtgttgctg tgcagctcct ccacgcggcg
       61 gaaggcgaac atggggcccc cgttctgcag gatgctgggg tggatggcgc tcttgaagtg
      121 catgtggctg tccaccacga agctgtagta gccgccgtcg cgcaggctga aggtgcgggc
      181 gaagctgccc accagcacgt tatcgcccat ggggtgcagg tgctccacgg tggcgttgct
      241 gcggatgatc ttgtcggtga agatcacgct gtcctcgggg aagccggtgc ccaccacctt
      301 gaagtcgccg atcacgcggc cggcctcgta gcggtagctg aagctcacgt gcagcacgcc
      361 gccgtcctcg tacttctcga tgcgggtgtt ggtgtagccg ccgttgttga tggcgtgcag
      421 gaaggggttc tcgtagccgc tggggtaggt gccgaagtgg tagaagccgt agcccatcac
      481 gtggctcagc aggtaggggc tgaaggtcag ggcgcctttg gtgctcttca tcttgttggt
      541 catgcggccc tgctcggggg tgccctctcc gccgcccacc agctcgaact ccacgccgtt
      601 cagggtgccg gtgatgcggc actcgatctt catggcgggc ataggaccgg ggttttcttc
      661 cacgtcccct gcttgcttta acagagagaa gttcgtggct ccggatccga cttttctttt
      721 tttcttggga tctaccttcc tcttcttttt tggatccacc ttcctcttct tcttggggtc
      781 agccctgctg tctccaccga gctgagagag gtcgattctt gtttcataga gccccgtaat
      841 tgactgatga
//
