LOCUS       sequence-158721-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of hsa-miR-27a-3p.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0 miR-27a-3p
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(74..787,788..790,791..793))
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(119..140)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     468..487
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     727..748
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(801..819)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(862..881)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          864..996
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
ORIGIN
        1 atgtaatgaa aataaagata ttttattgcg gccagcggcc ttcacagtgg ctaagttccg
       61 ctcgagcgat cgcttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
      121 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact
      181 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
      241 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
      301 gggccttgat gccgttcttc tgcttgtcgg cggtgatata gacgttgtgg ctgatgtagt
      361 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
      421 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
      481 cgtcgtcctt gaagaagatg gtacgctcct ggacgtagcc ttcgggcatg gcggacttga
      541 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccccaggtca
      601 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
      661 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacctgtggc
      721 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
      781 ccttgctcac cattggctag cctatagtga gtcgtattaa gtactctagc cttaagagct
      841 gtaattgaac tgggagtgga cacctgtgga gagaaaggca aagtggatgt cagtaagacc
      901 aataggtgcc tatcagaaac gcaagagtct tctctgtctc gacaagccca gtttctattg
      961 gtctccttaa acctgtcttg taaccttgat acttacctgc ccagtgcctc acgaccaact
     1021 tct
//
