LOCUS       sequence-158733-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cre recombinase alongside Cas9 and an 
            sgRNA cloning site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Walter DM, Venancio OS, Buza EL, Tobias JW, Deshpande C, Gudiel AA, 
            Kim-Kiselak C, Cicchini M, Yates TJ, Feldser DM
  TITLE     Systematic in vivo inactivation of chromatin regulating enzymes 
            identifies Setd2 as a potent tumor suppressor in lung 
            adenocarcinoma.
  JOURNAL   Cancer Res. 2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159.
  PUBMED    28202515
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer Res.
            2017 Feb 15. pii: canres.2159.2016. doi: 
            10.1158/0008-5472.CAN-16-2159."
COMMENT     Sequence Label: LentiCRISPRv2Cre
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..94
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             128..184
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             188..208
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggcctgtac gagacacgga tcgacctgtc tcagctggga ggcgacaagc gacctgccgc
       61 cacaaagaag gctggacagg ctaagaagaa gaaagattac aaagacgatg acgataaggg
      121 atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag agaatcctgg
      181 accgatgccc aagaagaaga ggaaggtgtc caatttactg accgtacacc aaaatttgcc
      241 tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag
      301 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg
      361 ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg
      421 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt
      481 gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc
      541 tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa
      601 acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag
      661 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt
      721 acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag
      781 aatgttaatc catattggca ga
//
