LOCUS       sequence-158739-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pACSA_J23113_tetR PEZ3_gfp.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Zess EK, Begemann MB, Pfleger BF
  TITLE     Construction of new synthetic biology tools for the control of gene 
            expression in the cyanobacterium Synechococcus sp. strain PCC 7002.
  JOURNAL   Biotechnol Bioeng. 2016 Feb;113(2):424-32. doi: 10.1002/bit.25713. 
            Epub 2015 Sep 3.
  PUBMED    26192329
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.25713";
            journalName: "Biotechnol Bioeng"; date: "2016-02"; volume: "113"; 
            issue: "2"; pages: "424-32"
COMMENT     Sequence Label: pEZ10
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             46..57
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(66..100)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 tgcgctgtta atcactttac ttttatctaa tctggacatc tagtatttct cctctttctc
       61 tagtagctag cataatccct aggactgagc tagccatcag gttaacaaaa aagcaggaat
      121 atccctatca gtgatagaca gacatcccta tcagtgatag agtataaagt taactgtggg
      181 attgcaaaag gagctcccaa ctcataaagt caagtaggag attaatcata tgtcggtcat
      241 caagcccgaa atgaaaatta aactgcgcat ggagggcgct gtgaacgggc acaagttcgt
      301 gatcgaaggt gaaggcattg gtaagccgta tgaaggtact caaaccctgg acttaacggt
      361 taaagaaggt gccccgttgc catttagcta cgacatcctg acacccgctt ttcagtatgg
      421 caatcgcgcg ttcaccaagt atccgaaaga tattccggat tatttcaagc aggcattccc
      481 ggaaggatac tcatgggaac gctcgatgac ctacgaagac cagggcattt gcattgccac
      541 aagcgacatc acaatggagg gtgattgctt tttttacaaa attcgcttcg acgggacaaa
      601 cttcccgccg aatggcccag tgatgcagaa aaagacgctt aaatgggaac cgtcgacaga
      661 gaaaatgtac gttcgcgatg gcgttctgaa aggcgatgtt aatatggcgc tgcttctcga
      721 agggggaggc cattaccgtt gtgatttcaa aaccacatac aaagcaaaaa aagatgttcg
      781 tttgccggac gcgcatgaag tcgatcaccg cattgagatc ctgtcacatg ataaagatta
      841 caataaggtc cgcctttatg a
//
