LOCUS       sequence-158747-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type MscL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Heureaux J, Chen D, Murray VL, Deng CX, Liu AP
  TITLE     Activation of a bacterial mechanosensitive channel in mammalian 
            cells by cytoskeletal stress.
  JOURNAL   Cell Mol Bioeng. 2014 Sep;7(3):307-319.
  PUBMED    25606062
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Bioeng."; date: "2014-09"; volume: "7(3)"; pages: "307-319"
COMMENT     Sequence Label: pADtet-His6-MscL
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             108..125
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             168..185
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(652..668)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..668)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(705..723)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    724..858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(761..780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     815..834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    867..900
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(912..930)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(913..930)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cccgaattcg ccaccatgag aggatctcac catcaccatc
      121 accatacgga tccgcatgcg agctcggtac cccgggtcga cctgcagctg gttccgcgtg
      181 gatccctcga gcatagggag aataacatga gcattattaa agaatttcgc gaatttgcga
      241 tgcgcgggaa cgtggtggat ttggcggtgg gtgtcattat cggtgcggca ttcgggaaga
      301 ttgtctcttc actggttgcc gatatcatca tgcctcctct gggcttatta attggcggga
      361 tcgattttaa acagtttgct gtcacgctac gcgatgcgca gggggatatc cctgctgttg
      421 tgatgcatta cggtgtcttc attcaaaacg tctttgattt tctgattgtg gcctttgcca
      481 tctttatggc gattaagcta atcaacaaac tgaatcggaa aaaagaagaa ccagcagccg
      541 cacctgcacc aactaaagaa gaagtattac tgacagaaat tcgtgatttg ctgaaagagc
      601 agaataaccg ctcttaatca ggactcagat ccgtcgacct gcagcccggg ggatccacta
      661 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
      721 attaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      781 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
      841 tcttatcatg tctggatcat cgatccataa cttcgtataa tgtatgctat acgaagttat
      901 ccagatctgg ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
      961 t
//
