LOCUS       sequence-158748-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses wild type MscL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Heureaux J, Chen D, Murray VL, Deng CX, Liu AP
  TITLE     Activation of a bacterial mechanosensitive channel in mammalian 
            cells by cytoskeletal stress.
  JOURNAL   Cell Mol Bioeng. 2014 Sep;7(3):307-319.
  PUBMED    25606062
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Mol 
            Bioeng."; date: "2014-09"; volume: "7(3)"; pages: "307-319"
COMMENT     Sequence Label: pADtet-His6-MscL
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..74
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     111..127
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     111..127
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             complement(594..611)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(654..671)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(768..792)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(789..827)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    860..878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    902..920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    944..962
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaatta
       61 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta
      121 gtggatcccc cgggctgcag gtcgacggat ctgagtcctg attaagagcg gttattctgc
      181 tctttcagca aatcacgaat ttctgtcagt aatacttctt ctttagttgg tgcaggtgcg
      241 gctgctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
      301 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
      361 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaaatcgatc
      421 ccgccaatta ataagcccag aggaggcatg atgatatcgg caaccagtga agagacaatc
      481 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
      541 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgctc gagggatcca
      601 cgcggaacca gctgcaggtc gacccggggt accgagctcg catgcggatc cgtatggtga
      661 tggtgatggt gagatcctct catggtggcg aattcggggc cgcggaggct ggatcggtcc
      721 cggtgtcttc tatggaggtc aaaacagcgt ggatggcgtc tccaggcgat ctgacggttc
      781 actaaacgag ctctgcttat ataggcctcc caccgtacac gcctacctcg acccgggtac
      841 cgagctcgac tttcactttt ctctatcact gatagggagt ggtaaactcg actttcactt
      901 ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat cactgatagg
      961 gagtggtaaa ctcgacttt
//
