LOCUS       sequence-158754-         681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, B RBS: TCGAGA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2B
FEATURES             Location/Qualifiers
     source          1..681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcagctttcg ttaaattatc aaacttacgt tgagtgatta acttggcgtt tagaagttgt
       61 ctccaatagt ttttcatctt tttgactact tcttcacttg gaacgttatc cgatttacca
      121 cgatttttat cagaacgcgt taagacctta ttgtctattg aatcgtcttt aaggaaactt
      181 tgtggaacaa tggcatcgac atcataatca cttaaacgat taatatctaa ttcttggtcc
      241 acatacatgt ctcttccatt ttggagataa tagagataga gcttttcatt ttgcaattga
      301 gtattttcaa caggatgctc tttaagaatc tgacttccta attctttgat accttcttcg
      361 attcgtttca tacgctctcg cgaatttttc tggccctttt gagttgtctg attttcacgt
      421 gccatttcaa taacgatatt ttctggctta tgccgcccca ttactttgac caattcatca
      481 acaactttta cagtctgtaa aatacctttt ttaatagcag ggctaccagc taaatttgca
      541 atatgttcat gtaaactatc gccttgtcca gacacttgtg ctttttgaat gtcttcttta
      601 aatgtcaaac tatcatcatg gatcagctgc ataaaattgc gattggcaaa accatctgat
      661 ttcaaaaaat ctaatattgt t
//
