LOCUS       sequence-158755-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  TITLE     LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His6 MBP TEV LIC cloning vector (1M)
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(101..148)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     144..162
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(215..232)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(298..318)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(334..357)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
       61 gcgtcccatt cgccaatccg gatatagttc ctcctttcag caaaaaaccc ctcaagaccc
      121 gtttagaggc cccaaggggt tatgctagtt attgctcagc ggtggcagca gccaactcag
      181 cttcctttcg ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg
      241 cggccgcaag cttgtcgacg gcgctcgaat tcggatccgt tatccacttc caatattgga
      301 ttggaagtac aggttttcct cgatcccatt agtctgcgcg tctttcaggg cttcatcgac
      361 agtctgacga ccgctggcgg cgttgatcac cgcagtacgc acggcatacc agaaagcgga
      421 catctgcggg atgttcggca tgatttcacc tttctgggcg ttttccatag tggcggcaat
      481 acgtggatct ttcgccaact cttcctcgta agacttcagc gctacggcac ccagcggttt
      541 gtctttatta accgcttcca gaccttcatc agtcagcaga tagttttcga ggaactcttt
      601 tgccagctct ttgttcggac tggcggcgtt aatacctgcg ctcagcacgc caacgaacgg
      661 tttggatggt tgacccttga aggtcggcag taccgttaca ccataattca ctttgctggt
      721 gtcgatgttg gaccatgccc acgggccgtt gatggtcatc gctgtttcgc ctttattaaa
      781 ggcagcttct gcgatggagt aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc
      841 aaccaggaag gtcagacccg ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc
      901 gtacttgccg ttttcatact tgaacgcata acccccgtca gcagcaatca gcggccaggt
      961 gaagtacggt tcttgcaggt tgaa
//
