LOCUS       sequence-158757-       10651 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  WT1 targeting donor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Bao X, Lian X, Hacker TA, Schmuck EG, Qian T, Bhute VJ, Han T, Shi 
            M, Drowley L, Plowright A, Wang QD, Goumans MJ, Palecek SP
  TITLE     Long-term self-renewing human epicardial cells generated from 
            pluripotent stem cells under defined xeno-free conditions.
  JOURNAL   Nat Biomed Eng. 2016;1. pii: 0003. doi: 10.1038/s41551-016-0003. 
            Epub 2016 Dec 5.
  PUBMED    28462012
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Biomed 
            Eng."; date: "2016"; volume: "1. pii: 0003. doi"; pages: " 
            10.1038/s41551-016-0003. Epub 2016 Dec 5"
COMMENT     Sequence Label: WT1-2A-eGFP PGK PuroR Donor Plasmid
FEATURES             Location/Qualifiers
     source          1..10651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(1298..1309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2302..2358
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(2359..2361,2362..2364,2365..3078)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2359..2361,2362..2364,2365..3075)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2404..2425)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2665..2684)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3012..3033
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3109..3142
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3203..3699
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3238..3261)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3632..3651
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3739..4338
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3739..3758)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4235..4255
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    4895..4928
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7084..7103)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7085..7103)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7110..7127)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7110..7126)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7119..7141)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7267..7695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7354..7373)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7564..7585
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             8039..8833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8093..8112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8703..8722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(8851..8919,8920..9711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9069..9088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9882..10470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10371..10390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10624..10641
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttttcctg
      301 cagggctgtg atcccaccac tgtactccaa cccaagcaac agagcaagac cctgtctcaa
      361 aacaaaacaa aacaaaacaa acaaaaaaaa atgaggtagg catgttttat tcccatctta
      421 cagatgagga gactgaggca ataataattc aatggcttat ctaaagtcac aaagctagta
      481 aggagcaaat ccagttctgt ctgcttccag cccaccttgt cccacttgct tctttattgg
      541 agaaggggca gatatttact ctgcagtcca gggagcatgg agagtcaagt agagaatcaa
      601 agtcagcaaa gctgaacacc actgagagac tgagaggaat gaagatgaca agagacaggg
      661 ttgggattag tccattagga gccagccctt aacagcactg ctggaggtgg ggttggagag
      721 tgagagccag gccaggaaga gcagaggaac agcgagtgaa ggaaaacatg acatcacaaa
      781 cccctgagca gccccagatt cttcgcccaa attggctgca tttaaaaaca gagccaaggc
      841 acctgtggcc catggttggc aggatgggat cttgcttccc ttcctcaggt gcctttgatg
      901 cttctttaac ttagtcctga gctcataatt cggtggcaaa gtgttatagt ccctttgaca
      961 cggctgtcac actccccaag taagtgtgac atgacctgtt ttgccagcag actgtttaat
     1021 accacatttg attggcctcc agtctgaccc tgggacacca gccctcctgg gacatttctc
     1081 agtgcttgtc caaaccagta gttctcagag aaggccaagt gggaccagca gtatctgaga
     1141 agatccgtca ggtggcacag ggtttagctt ctgtgcattc ttccttaaga tggagctgca
     1201 gctgcttttc catcatggct aggcctggga ggtgtgatac agggaagagc acgggctctg
     1261 gaattcgaca gacctggaga tttgagttct gcctggatct gccctcaata gatgtcctac
     1321 ctcatctgta gagtggggat aataacgctt cccctacggg cagttgtaag gagtacatga
     1381 ggttgtgtgt gtgcgtgtgt gaagcccagc acaaggcttg gcatatgtta agcatcaaat
     1441 aaatggtagt tattctccct aatgagaatg aggcttggaa agaatgtctg agggctcttt
     1501 gggtggagaa gcctcaatct tttggcaagc ttattaaaaa tctaagaacc acgccccaac
     1561 tagaatcttc catataacag gattatgctc tcagtaacac tttaagagaa aaaaaaaaaa
     1621 caacacataa catgtaaact ggtatcagtg atttatttta aagtatccag cccatccagg
     1681 gtatctacac agatagaagt gttaaaagct tcagtcacag ctgagatccc tggacagtga
     1741 tcactcagta atacccaggg tagatgaccc agaggcttct gcagcccagt tcatctcagt
     1801 tcccaactta ctcaacaagg gggagaaatt tacacagttc acaaaacatt ctatcagcag
     1861 agactgaaac aaacgttttt ccccttaatt atgaattgct cagacatttc ccccaacttg
     1921 actcattctt tatctcccac tttccaagtt ttctgaccta agaatataac tttttacaat
     1981 atttcgatcc ttaaagcccc aagaggtgct gctgtttcaa ggctgcctgg gaaatgagct
     2041 cagttggtta gctcagggac agaatgatgg gaaattcaga gtgggtgcct tgtgatgact
     2101 tcactcgggc cttgatagtt gaacttgcgc ctgtctcttt gttgcaagtg tctctgactg
     2161 gcaattgtgt caacaggtga aaagcccttc agctgtcggt ggccaagttg tcagaaaaag
     2221 tttgcccggt cagatgaatt agtccgccat cacaacatgc atcagagaaa catgaccaaa
     2281 ctccagctgg cgcttgctag cgccactaac ttctccctgt tgaaacaagc aggggatgtc
     2341 gaagagaatc ccgggccaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2401 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2461 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2521 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2581 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2641 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2701 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2761 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2821 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2881 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2941 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     3001 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     3061 gacgagctgt acaagtaaga attccgatca tattcaataa cccttaatat aacttcgtat
     3121 aatgtatgct atacgaagtt attaggtctg aagaggagtt tacgtccagc caagcttagg
     3181 atctcgacct cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca
     3241 tgcgctttag cagccccgct ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3301 attccacatc caccggtagc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     3361 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     3421 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     3481 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     3541 ggctcagcag ctgggaaggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     3601 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     3661 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcatcca tctagatctc
     3721 gagcagctga agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     3781 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     3841 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     3901 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     3961 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     4021 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     4081 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     4141 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     4201 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     4261 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     4321 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     4381 cgaccccatg catcgatgat atcagatccc cgggatgcag aaattgatga tctattaaac
     4441 aataaagatg tccactaaaa tggaagtttt tcctgtcata ctttgttaag aagggtgaga
     4501 acagagtacc tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga
     4561 ttagataaat gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca
     4621 tagttggata tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca
     4681 ctcatgatct atagatctat agatctctcg tgggatcatt gtttttctct tgattcccac
     4741 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
     4801 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
     4861 catcagaagc tggtcgagat ccggaaccct taatataact tcgtataatg tatgctatac
     4921 gaagttatta ggtccctcga agaggttcac taggcgcgcc gttcagtgtc ccaggcagca
     4981 cagtgtgtga actgctttca agtctgactc tccactcctc ctcactaaaa aggaaacttc
     5041 agttgatctt cttcatccaa cttccaagac aagataccgg tgcttctgga aactaccagg
     5101 tgtgcctgga agagttggtc tctgccctgc ctacttttag ttgactcaca ggccctggag
     5161 aagcagctaa caatgtctgg ttagttaaaa gcccattgcc atttggtgtg gattttctac
     5221 tgtaagaaga gccatagctg atcatgtccc cctgaccctt cccttctttt tttatgctcg
     5281 ttttcgctgg ggatggaatt attgtaccat tttctatcat ggaatattta taggccaggg
     5341 catgtgtatg tgtctgctaa tgtaaacttt gtcatggttt ccatttacta acagcaacag
     5401 caagaaataa atcagagagc aaggcatcgg gggtgaatct tgtctaacat tcccgaggtc
     5461 agccaggctg ctaacctgga aagcaggatg tagttctgcc aggcaacttt taaagctcat
     5521 gcatttcaag cagctgaaga aaaaatcaga actaaccagt acctctgtat agaaatctaa
     5581 aagaatttta ccattcagtt aattcaatgt gaacactggc acactgctct taagaaacta
     5641 tgaagatctg agattttttt gtgtatgttt ttgactcttt tgagtggtaa tcatatgtgt
     5701 ctttatagat gtacatacct ccttgcacaa atggagggga attcattttc atcactggga
     5761 gtgtccttag tgtataaaaa ccatgctggt atatggcttc aagttgtaaa aatgaaagtg
     5821 actttaaaag aaaatagggg atggtccagg atctccactg ataagactgt ttttaagtaa
     5881 cttaaggacc tttgggtcta caagtatatg tgaaaaaaat gagacttact gggtgaggaa
     5941 atccattgtt taaagatggt cgtgtgtgtg tgtgtgtgtg tgtgtgtgtg tgtgttgtgt
     6001 tgtgttttgt tttttaaggg agggaattta ttatttaccg ttgcttgaaa ttactgtgta
     6061 aatatatgtc tgataatgat ttgctctttg acaactaaaa ttaggactgt ataagtacta
     6121 gatgcatcac tgggtgttga tcttacaaga tattgatgat aacacttaaa attgtaacct
     6181 gcatttttca ctttgctctc aattaaagtc tattcaaaag gaaagtggca agattccatc
     6241 agctgtattg tgttcaaatc tcaaggccac tgagcctctt ttaaacaggt aagagaagac
     6301 gccaccgaca tcgacggcta ctagtgagcc tatccaggtc ctgaccacaa tttatttctc
     6361 tctgtcaaac ttcaggaggg acttatatgt cctagaagca acagagaata tcttcacctt
     6421 gtgttacaga gtgattgtgc tcatgctgtg gttgaatggg gtttgtggat gatgtggtga
     6481 ttatgtttta aggttttaag gtttatttta agcagattgt cattgcatcc catcatccca
     6541 gatcagcaaa agaaaataga gagatgttaa tgacaactag tccttttgaa aagttcagat
     6601 acatcactgg gagggcatag gctgaacacc tagtacaatt gaatttacag ctgtacttgc
     6661 tataaatcag ctcagcagat tgaaaactgc tcagcctttc cagggcaact gagtgtgaaa
     6721 gcaaagatct atattatcta taaactataa actctctttc tgttctggtg tatggttttt
     6781 gaattgtaaa taaggagatt aatttggtgc agcccctctc agctccatta tcttggggct
     6841 tgcatgcatt ccgggtttta tttcttcatt taaaatgcgt ctcaaacaga tggaagccta
     6901 gctatggaga ctgttttaca ttgaagtgca gctcaaagtt tgggcagcct aaaagtcagg
     6961 tccagaggcc cctcttattt tgcatctggc tcttgcatca ctgtggccgg ccaaaagggc
     7021 gaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggcccaat
     7081 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
     7141 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7201 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7261 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7321 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7381 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7441 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7501 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7561 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7621 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7681 aatttaacgc gaattttaac aaaattcagg gcgcaagggc tgctaaagga agcggaacac
     7741 gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct actgggctat
     7801 ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg ggcttacatg
     7861 gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc cagctggggc
     7921 gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag
     7981 gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat cgtttcgcat
     8041 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     8101 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     8161 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     8221 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     8281 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     8341 tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     8401 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     8461 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     8521 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     8581 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     8641 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     8701 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     8761 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     8821 cgagttcttc tgaattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     8881 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     8941 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9001 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9061 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9121 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9181 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9241 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     9301 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     9361 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     9421 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     9481 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     9541 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     9601 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     9661 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     9721 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     9781 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     9841 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9901 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9961 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10021 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10081 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10141 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10201 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10261 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10321 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10381 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10441 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10501 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10561 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10621 cgcagcgagt cagtgagcga ggaagcggaa g
//
