LOCUS       sequence-158759-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2C
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..42
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(51..85)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 tttactttta tctaatctgg acatctagta tttctcctct ttctctagta gctagcataa
       61 tccctaggac tgagctagcc atcaggttaa caaaaaagca ggaatatccc tatcagtgat
      121 agacagacat ccctatcagt gatagagtat aaagttaact gtgggattgc aaaaggagct
      181 cccaactcat aaagtcaagt tggacattaa ttccatggat aagaaatact caataggctt
      241 agctatcggc acaaatagcg tcggatgggc ggtgatcact gatgaatata aggttccgtc
      301 taaaaagttc aaggttctgg gaaatacaga ccgccacagt atcaaaaaaa atcttatagg
      361 ggctctttta tttgacagtg gagagacagc ggaagcgact cgtctcaaac ggacagctcg
      421 tagaaggtat acacgtcgga agaatcgtat ttgttatcta caggagattt tttcaaatga
      481 gatggcgaaa gtagatgata gtttctttca tcgacttgaa gagtcttttt tggtggaaga
      541 agacaagaag catgaacgtc atcctatttt tggaaatata gtagatgaag ttgcttatca
      601 tgagaaatat ccaactatct atcatctgcg aaaaaaattg gtagattcta ctgataaagc
      661 ggatttgcgc ttaatctatt tggcctta
//
