LOCUS       sequence-158760-       15380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET probe for measuring tension on Talin molecule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15380)
  AUTHORS   Kumar A, Ouyang M, Van den Dries K, McGhee EJ, Tanaka K, Anderson 
            MD, Groisman A, Goult BT, Anderson KI, Schwartz MA
  TITLE     Talin tension sensor reveals novel features of focal adhesion force 
            transmission and mechanosensitivity.
  JOURNAL   J Cell Biol. 2016 May 9;213(3):371-83. doi: 10.1083/jcb.201510012.
  PUBMED    27161398
REFERENCE   2  (bases 1 to 15380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-05-9- 9"; volume: "213"; 
            issue: "3"; pages: "371-83"
COMMENT     Sequence Label: Talin-TS
FEATURES             Location/Qualifiers
     source          1..15380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1566..2165
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1566..1585)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2062..2082
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2340..2643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2644..2847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2798..2818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(4259..4261,4262..4264,4265..4777)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(4304..4325)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4565..4584)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4912..4933
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5069..5779
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     LTR             12119..12712
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(12815..12837)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    12965..12998
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    13058..13091
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     13111..13130
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13211..13228)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13382..13970)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13462..13481)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14141..14932,14933..15001))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14764..14783
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15002..15106)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15174..15192
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2881 cggactcaga tctcgaagct tcgaattcac catggttgcg ctttcgctga agattagcat
     2941 tgggaatgtg gtgaagacga tgcaatttga gccatctacc atggtgtacg acgcctgccg
     3001 catgattcgt gagcggatcc cagaggccct ggctggccct cccaacgact ttgggctctt
     3061 tctgtcagat gatgacccca aaaaaggcat ctggctagag gctgggaaag ctttggacta
     3121 ctacatgctc cgaaatgggg acaccatgga gtacagaaaa aagcagagac ccctgaagat
     3181 ccggatgtta gacggaacgg tgaagactat catggtggat gactccaaga ccgtcacaga
     3241 catgctcatg accatttgtg cccgaattgg tatcaccaac catgatgagt attcactggt
     3301 tcgagagctg atggaagaaa agaaagatga ggggacaggg accctgagaa aagacaagac
     3361 cctactgcga gatgaaaaga agatggagaa gctaaagcag aagttgcaca cagacgatga
     3421 gttgaactgg ctggaccatg ggcggacact gagggaacag ggagtggaag agcatgagac
     3481 gttgctgctg cggagaaagt tcttctactc agaccagaat gtggattccc gagaccctgt
     3541 acagctgaac cttctctatg tgcaggcacg agatgacatc ctgaatggct cccatcctgt
     3601 ctcctttgac aaggcctgtg aatttgcagg cttccagtgc cagatccagt ttggacctca
     3661 caatgaacag aagcacaagg ctggcttcct tgacctgaag gacttcctgc ccaaggagta
     3721 tgtgaagcag aagggagagc gtaagatctt tcaggcacac aagaattgtg ggcagatgag
     3781 tgaaattgag gccaaggtac gctacgtgaa gctggcccgt tccctcaaga cttacggtgt
     3841 ctccttcttc ctagtcaagg aaaagatgaa ggggaagaat aaactggtgc ccaggctgtt
     3901 gggcatcact aaggagtgtg tgatgcgtgt ggatgagaag accaaggagg tgattcagga
     3961 atggagcctc accaacatca aacgctgggc tgcctctccc aagagcttta ctctggactt
     4021 tggagactat caggatggct actactcagt acagacgaca gaaggcgagc agatcgcaca
     4081 gctcattgct ggctacatag atatcatcct taagaagaaa aaaagcaagg accattttgg
     4141 gctggaggga gatgaagagt ctactatgtt ggaggactca gtgtctccca aaaagtcaac
     4201 agtccttcag cagcagtaca accgagtggg gaaagtggag cacggctctg tggtcgagat
     4261 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     4321 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     4381 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     4441 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     4501 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     4561 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     4621 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     4681 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     4741 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     4801 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     4861 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     4921 gctggagttc gtgaccgccg ccggggggcc cggtggtgca ggaccaggtg gtgcaggtcc
     4981 aggtggtgca ggaccaggtg gtgcaggacc tggtggtgca ggtccaggtg gtgcaggtcc
     5041 aggtggtgca ggtccaggtg gtgctcatat ggtgtctaag ggcgaagagc tgattaagga
     5101 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
     5161 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
     5221 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag
     5281 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccctgaggg
     5341 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     5401 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
     5461 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct
     5521 gtaccccgct gacggcggcc tggaaggcag aagcgacatg gccctgaagc tcgtgggcgg
     5581 gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct
     5641 caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa
     5701 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     5761 actggggcac aaacttaatg cggccgcggc tctgccagcc atcatgcgct ctggagcctc
     5821 tggtcctgag aatttccagg tggggagcat gccacctgcc cagcagcaga tcaccagtgg
     5881 ccagatgcac cgaggacaca tgccacctct gacttcagcc cagcaggcgc tcactggaac
     5941 cattaactcc agcatgcagg ctgtgcaggc tgcccaggcc actctggatg actttgagac
     6001 tctaccccct cttggccagg atgctgcctc caaagcttgg cgtaagaaca agatggatga
     6061 atcaaagcat gagatccact cccaggtaga tgccatcaca gctggcactg cctctgtggt
     6121 aaacctgaca gcaggagatc ctgcagagac agactataca gcagtgggct gtgcagtcac
     6181 caccatctct tccaacctga cggagatgtc ccgtggggtg aagctattgg ctgccctgct
     6241 ggaggatgaa ggcggcaatg gccggcccct tctgcaagca gcaaagggcc ttgcaggggc
     6301 tgtgtcggaa ctgcttcgca gtgctcagcc cgccagcgct gagcctcgtc agaacctgct
     6361 gcaggcagcc gggaacgtgg gccaggccag tggggagctg ttgcagcaaa ttggggaaag
     6421 tgacactgac ccccacttcc aggatgttct aatgcagcta gcaaacgcag tggcaagtgc
     6481 tgcagccgcc ctggtcctca aggccaagag tgtggcccag cgcacagagg attccgggct
     6541 tcagacccaa gtgattgctg cagctacgca atgtgctctg tccacttccc agctggtggc
     6601 ctgtaccaag gtggtagcac ctacaatcag ctcacctgtg tgccaagaac agctagtcga
     6661 ggctgggcga ctggtggcca aagctgtgga gggctgtgtg tctgcctcgc aagcagctac
     6721 agaagatgga cagctgcttc gaggggtagg agcagcagcc acggctgtca cccaggccct
     6781 caatgagctg ctgcagcacg tgaaggccca cgccaccgga gctgggcctg ccggtcggta
     6841 tgaccaagcc actgatacca tcctcaccgt cactgaaaac atcttcagct ccatgggtga
     6901 tgctggggag atggtgcgcc aggcccgcat cctggcccaa gccacctcag acctggtcaa
     6961 tgctatcaag gccgatgctg agggggagag tgatctggag aactctagaa agctcctgag
     7021 tgctgccaag atcctcgctg atgccaccgc caagatggtg gaggcggcca agggagcagc
     7081 cgcccaccct gacagtgagg aacagcagca gcgactgcgt gaagcagctg aggggcttcg
     7141 catggccacc aatgcagctg cgcagaacgc catcaagaag aagttggtgc agcgcctgga
     7201 gcatgcagcc aagcaagctg cagcctctgc aacacagacc attgctgcag cccaacatgc
     7261 agcctctgcc cccaaggcct ctgcgggccc ccagccccta ctggtgcaga gctgtaaggc
     7321 cgtggcagag cagattccgt tgctggtgca gggtgtccga ggaagtcaag ctcagcctga
     7381 cagccctagt gctcagctcg ccctcattgc tgccagccag agcttcctgc agccaggtgg
     7441 gaagatggtg gcagcggcaa aagcctcagt gccaacaatt caggaccagg cttcagcgat
     7501 gcagctgagt cagtgcgcaa agaacctagg cactgccctg gccgagctgc gcactgctgc
     7561 tcagaaggct caggaggcat gtggaccttt ggagatggat tctgcactga gtgttgtaca
     7621 aaatctagag aaagacctac aggaaataaa agcagcagct cgagatggca agcttaagcc
     7681 cttacctggg gaaacaatgg agaagtgtac ccaagatctt ggcaacagca ccaaagcggt
     7741 gagctctgcc atcgccaagc tgctggggga gattgcccag ggcaatgaga actatgcagg
     7801 tattgcagct cgggatgtag ctggtggact aaggtcacta gcccaggctg cgcggggtgt
     7861 ggctgcgctg acatcagatc ctgcagtgca ggccattgtg cttgacacag ccagcgacgt
     7921 tctggacaag gccagcagcc tcattgaaga ggcaaaaaaa gcatctggac acccagggga
     7981 cccagaaagc cagcaaaggc ttgctcaggt agctaaagca gtgacccaag ccctgaaccg
     8041 ctgtgtcagc tgcctgcctg gccaaagaga cgtggataat gccctacgag ccgttggaga
     8101 tgccagcaag cgactcctga gtgacttgct tcctccaagc acggggacat ttcaagaagc
     8161 acagagccga ttgaatgaag ctgcagctgg gttaaatcag gccgccacag aactggtgca
     8221 agcctcccga ggaactcctc aggacctggc tcgggcctcc ggccgatttg gacaggactt
     8281 cagcaccttc ctggaagctg gtgtggagat ggccggacag gccccgagcc aggaggaccg
     8341 agcccaggtg gtgtccaacc tgaagggcat atccatgtct tcaagcaaac ttctccttgc
     8401 cgctaaggcc ttgtccacag accctgcttc tcccaacctc aagagtcagc tggctgcggc
     8461 tgcccgggca gtgacggaca gcatcaacca gctcatcacc atgtgcaccc agcaggcacc
     8521 tggccagaag gagtgtgaca atgcacttcg gcagctagag acagtccgag aactcctgga
     8581 gaatccagtc cagcccatca acgacatgtc ctacttcggt tgcttggaca gtgtcatgga
     8641 gaactccaag gtcctaggtg aggccatgac tggcatctcc caaaatgcca agaatggaaa
     8701 tctgccggag tttggagatg ccattgccac agcctccaag gctctctgtg gcttcacgga
     8761 ggcagccgca caggcagcat atctagttgg tgtctctgac cccaacagcc aagctggaca
     8821 gcaaggactg gtggaaccca cacagtttgc ccgtgcaaac caggcaattc agatggcctg
     8881 tcagagtctg ggggagcctg gctgtaccca ggcccaggtg ttatctgcag ccactattgt
     8941 agccaaacac acatctgcat tgtgtaacag ctgtcgcctg gcttccgcta ggactgccaa
     9001 tcccactgcc aagcgccagt ttgtacagtc agccaaggag gtggccaaca gtacagccaa
     9061 tcttgtcaag accatcaagg cactagatgg ggacttcaca gaagagaacc gtgcccagtg
     9121 ccgagcagcc acagcccctc tgctggaagc tgtggataac ctgagtgcct ttgcctccaa
     9181 ccctgagttc tccagcgtcc ctgcccagat cagccctgag ggcagagcag ccatggagcc
     9241 cattgtaatc tctgctaaga caatgttgga gagtgctgga gggctcatcc agacagctcg
     9301 ggcgttagca gtcaatcccc gagacccccc acgttggtct gtgctagctg gccactctcg
     9361 gactgtctca gattccatca agaaacttat tacaagcatg agggacaaag ccccagggca
     9421 gctggagtgt gagacagcca ttgcggctct gaacagctgc ttgcgggacc tagatcaggc
     9481 ttcgcttgcc gctgtcagcc agcagcttgc tccccgtgaa ggaatctctc aagaggcttt
     9541 gcacacccag atgctcactg cagtgcaaga aatttctcat ctcattgagc cgctggccag
     9601 cgctgctcgg gctgaagcct cccagctggg acacaaggtg tcccaaatgg cccagtactt
     9661 tgaaccactc accctggctg cagtgggtgc tgcgtctaag accctgagcc acccgcagca
     9721 gatggcactt ctggaccaga ctaaaacgtt ggcagagtct gccttgcagt tgctatatac
     9781 tgccaaggag gctggtggta accccaagca agcagcacac acccaggaag ccctggagga
     9841 ggctgtgcag atgatgacag aggccgtaga agacctgacg acgaccctca acgaagcagc
     9901 cagtgctgca ggagtcgttg gcggcatggt ggactctatc actcaggcca tcaaccagct
     9961 agatgaagga cctatgggtg acccagaagg ctcattcgta gattaccaga caaccatggt
    10021 gaggacagcc aaggccattg ctgtcactgt tcaggagatg gtaaccaagt caaacaccag
    10081 ccctgaagag ctaggccctc ttgccaacca gctgaccagt gactatggcc gactggcctc
    10141 acaagccaag cctgcagctg tggctgctga aaatgaagag ataggcgctc acatcaaaca
    10201 ccgagtacag gagctgggcc atggctgctc tgctctggtc accaaggcag gtgccttgca
    10261 gtgtagcccc agtgatgtct acaccaagaa ggagctcata gagtgtgccc gcagagtgtc
    10321 agagaaggtc tcccatgttc tggctgcact ccaggctggg aatcgtggta cccaggcctg
    10381 cattacagca gccagtgctg tgtctggtat cattgctgac ctcgacacca ccatcatgtt
    10441 cgctactgct ggcacactta accgtgaggg tgctgaaact tttgctgacc accgggaggg
    10501 tatcttaaag acagcaaagg ttcttgtgga ggacaccaag gtcctagtgc agaatgcagc
    10561 tgggagccag gagaagttgg cacaagccgc ccagtcctcc gtggccacca ttacccgcct
    10621 cgctgatgtg gtcaagctcg gtgcagccag cctaggagcc gaagaccctg aaactcaggt
    10681 ggtgctgatc aatgcagtaa aggacgtagc caaggccctg ggtgacctca tcagcgctac
    10741 gaaggctgca gcgggcaaag ttggggatga ccctgcagtg tggcagctca agaactctgc
    10801 caaggtgatg gtgaccaatg tgacatcatt gctcaagaca gtgaaggctg tggaagatga
    10861 ggccaccaaa ggcacacggg ccctagaggc aaccacagag cacatacgtc aggaactggc
    10921 ggtcttctgt tccccagagc cacctgccaa gacctctacc cctgaagact tcatccgaat
    10981 gaccaagggt attactatgg caacagccaa agccgttgct gctggcaatt cctgtcgaca
    11041 ggaagatgtc attgccacag ccaatctgag ccgacgggct attgcggaca tgcttcgggc
    11101 ttgcaaggaa gcagctttcc acccagaagt ggcgcctgat gtacggctcc gagccctgca
    11161 ctatggccgg gagtgtgcca atggttacct ggaactgctg gaccacgtgc tgctgaccct
    11221 tcagaagcca aacccagacc tgaagcagca gctaactgga cactcaaagc gggttgctgg
    11281 ctctgtgact gagctcatcc aggctgctga agccatgaaa ggaacagagt gggtggaccc
    11341 agaggaccct actgtcattg ctgagaatga gctcttggga gccgcagccg ccatcgaggc
    11401 tgcagccaag aagctggagc agctgaagcc cagggccaaa cccaaggagg cggatgagtc
    11461 cttgaacttt gaggaacaaa tcctagaagc tgccaagtcc atcgctgcag ccaccagtgc
    11521 actggtaaag gctgcgtcag cagcccagag ggagctggtg gctcaaggaa aggtgggcgc
    11581 cattccagcc aatgcactgg atgatgggca gtggtcgcag ggcctcattt ccgctgcccg
    11641 tatggtggct gcggccacca acaatctgtg tgaagcagct aacgcagctg tccagggcca
    11701 tgctagccag gagaaactca tttcctcagc caagcaagta gctgcctcca cagctcagct
    11761 cctggtagct tgcaaggtca aagcagatca ggactctgag gcaatgaaac ggctccaggc
    11821 tgctggcaat gcagtgaaga gggcctcaga taacctggta aaggcggccc agaaagctgc
    11881 agccttcgaa gaccaggaga atgagacggt ggtggtgaag gagaaaatgg ttgggggcat
    11941 tgcccagatt atcgcagcac aggaagagat gcttcggaag gaacgagagc tggaagaggc
    12001 tcggaaaaag ctcgcccaga tccggcagca gcagtacaag ttcttgcctt cagagcttcg
    12061 agacgagcac taaatcgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
    12121 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
    12181 ggaaaaatac ataactgaga atagagaagt tcagatcaag gtcaggaaca gatggaacag
    12241 ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
    12301 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
    12361 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
    12421 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
    12481 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
    12541 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
    12601 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
    12661 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
    12721 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
    12781 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
    12841 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
    12901 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
    12961 actgataact tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg
    13021 tccagccaag ctagggccgc gatccggaac ccttaatata acttcgtata atgtatgcta
    13081 tacgaagtta tcagtactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
    13141 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
    13201 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    13261 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    13321 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    13381 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    13441 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    13501 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    13561 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    13621 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    13681 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    13741 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    13801 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
    13861 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    13921 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    13981 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    14041 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    14101 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    14161 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    14221 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    14281 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    14341 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    14401 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
    14461 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    14521 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    14581 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    14641 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    14701 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
    14761 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    14821 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    14881 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    14941 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    15001 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    15061 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    15121 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    15181 gtatcacgag gccctttcgt cttcaagaat tgctagcaat tgctagcaat tgctagcaat
    15241 tcataccaga tcaccgaaaa ctgtcctcca aatgtgtccc cctcacactc ccaaattcgc
    15301 gggcttctgc ctcttagacc actctaccct attccccaca ctcaccggag ccaaagccgc
    15361 ggcccttccg tttctttgct
//
