LOCUS       sequence-158762-       15416 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control FRET probe for measuring tension on Talin molecule.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15416)
  AUTHORS   Kumar A, Ouyang M, Van den Dries K, McGhee EJ, Tanaka K, Anderson 
            MD, Groisman A, Goult BT, Anderson KI, Schwartz MA
  TITLE     Talin tension sensor reveals novel features of focal adhesion force 
            transmission and mechanosensitivity.
  JOURNAL   J Cell Biol. 2016 May 9;213(3):371-83. doi: 10.1083/jcb.201510012.
  PUBMED    27161398
REFERENCE   2  (bases 1 to 15416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2016-05-9- 9"; volume: "213"; 
            issue: "3"; pages: "371-83"
COMMENT     Sequence Label: Talin-CS
FEATURES             Location/Qualifiers
     source          1..15416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             414..589
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    652..851
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1052..1468
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1398..1420
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1436..1452
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1566..2165
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(1566..1585)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2062..2082
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        2340..2643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2644..2847
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2798..2818
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(10586..10588,10589..10591,10592..11104)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(10631..10652)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10892..10911)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11239..11260
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             11396..12109
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     LTR             12155..12748
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(12851..12873)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    13001..13034
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    13094..13127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     13147..13166
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13247..13264)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13418..14006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13498..13517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14177..14968,14969..15037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14800..14819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15038..15142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15210..15228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
       61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
      121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
      181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
      241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
      301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
      361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
      421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
      481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
      541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
      601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
      721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
      841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
      901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
      961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
     1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
     1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
     1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
     1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
     1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
     1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
     1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
     1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
     1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
     1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
     2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
     2881 cggactcaga tctcgaagct tcgaattcac catggttgcg ctttcgctga agattagcat
     2941 tgggaatgtg gtgaagacga tgcaatttga gccatctacc atggtgtacg acgcctgccg
     3001 catgattcgt gagcggatcc cagaggccct ggctggccct cccaacgact ttgggctctt
     3061 tctgtcagat gatgacccca aaaaaggcat ctggctagag gctgggaaag ctttggacta
     3121 ctacatgctc cgaaatgggg acaccatgga gtacagaaaa aagcagagac ccctgaagat
     3181 ccggatgtta gacggaacgg tgaagactat catggtggat gactccaaga ccgtcacaga
     3241 catgctcatg accatttgtg cccgaattgg tatcaccaac catgatgagt attcactggt
     3301 tcgagagctg atggaagaaa agaaagatga ggggacaggg accctgagaa aagacaagac
     3361 cctactgcga gatgaaaaga agatggagaa gctaaagcag aagttgcaca cagacgatga
     3421 gttgaactgg ctggaccatg ggcggacact gagggaacag ggagtggaag agcatgagac
     3481 gttgctgctg cggagaaagt tcttctactc agaccagaat gtggattccc gagaccctgt
     3541 acagctgaac cttctctatg tgcaggcacg agatgacatc ctgaatggct cccatcctgt
     3601 ctcctttgac aaggcctgtg aatttgcagg cttccagtgc cagatccagt ttggacctca
     3661 caatgaacag aagcacaagg ctggcttcct tgacctgaag gacttcctgc ccaaggagta
     3721 tgtgaagcag aagggagagc gtaagatctt tcaggcacac aagaattgtg ggcagatgag
     3781 tgaaattgag gccaaggtac gctacgtgaa gctggcccgt tccctcaaga cttacggtgt
     3841 ctccttcttc ctagtcaagg aaaagatgaa ggggaagaat aaactggtgc ccaggctgtt
     3901 gggcatcact aaggagtgtg tgatgcgtgt ggatgagaag accaaggagg tgattcagga
     3961 atggagcctc accaacatca aacgctgggc tgcctctccc aagagcttta ctctggactt
     4021 tggagactat caggatggct actactcagt acagacgaca gaaggcgagc agatcgcaca
     4081 gctcattgct ggctacatag atatcatcct taagaagaaa aaaagcaagg accattttgg
     4141 gctggaggga gatgaagagt ctactatgtt ggaggactca gtgtctccca aaaagtcaac
     4201 agtccttcag cagcagtaca accgagtggg gaaagtggag cacggctctg tggctctgcc
     4261 agccatcatg cgctctggag cctctggtcc tgagaatttc caggtgggga gcatgccacc
     4321 tgcccagcag cagatcacca gtggccagat gcaccgagga cacatgccac ctctgacttc
     4381 agcccagcag gcgctcactg gaaccattaa ctccagcatg caggctgtgc aggctgccca
     4441 ggccactctg gatgactttg agactctacc ccctcttggc caggatgctg cctccaaagc
     4501 ttggcgtaag aacaagatgg atgaatcaaa gcatgagatc cactcccagg tagatgccat
     4561 cacagctggc actgcctctg tggtaaacct gacagcagga gatcctgcag agacagacta
     4621 tacagcagtg ggctgtgcag tcaccaccat ctcttccaac ctgacggaga tgtcccgtgg
     4681 ggtgaagcta ttggctgccc tgctggagga tgaaggcggc aatggccggc cccttctgca
     4741 agcagcaaag ggccttgcag gggctgtgtc ggaactgctt cgcagtgctc agcccgccag
     4801 cgctgagcct cgtcagaacc tgctgcaggc agccgggaac gtgggccagg ccagtgggga
     4861 gctgttgcag caaattgggg aaagtgacac tgacccccac ttccaggatg ttctaatgca
     4921 gctagcaaac gcagtggcaa gtgctgcagc cgccctggtc ctcaaggcca agagtgtggc
     4981 ccagcgcaca gaggattccg ggcttcagac ccaagtgatt gctgcagcta cgcaatgtgc
     5041 tctgtccact tcccagctgg tggcctgtac caaggtggta gcacctacaa tcagctcacc
     5101 tgtgtgccaa gaacagctag tcgaggctgg gcgactggtg gccaaagctg tggagggctg
     5161 tgtgtctgcc tcgcaagcag ctacagaaga tggacagctg cttcgagggg taggagcagc
     5221 agccacggct gtcacccagg ccctcaatga gctgctgcag cacgtgaagg cccacgccac
     5281 cggagctggg cctgccggtc ggtatgacca agccactgat accatcctca ccgtcactga
     5341 aaacatcttc agctccatgg gtgatgctgg ggagatggtg cgccaggccc gcatcctggc
     5401 ccaagccacc tcagacctgg tcaatgctat caaggccgat gctgaggggg agagtgatct
     5461 ggagaactct agaaagctcc tgagtgctgc caagatcctc gctgatgcca ccgccaagat
     5521 ggtggaggcg gccaagggag cagccgccca ccctgacagt gaggaacagc agcagcgact
     5581 gcgtgaagca gctgaggggc ttcgcatggc caccaatgca gctgcgcaga acgccatcaa
     5641 gaagaagttg gtgcagcgcc tggagcatgc agccaagcaa gctgcagcct ctgcaacaca
     5701 gaccattgct gcagcccaac atgcagcctc tgcccccaag gcctctgcgg gcccccagcc
     5761 cctactggtg cagagctgta aggccgtggc agagcagatt ccgttgctgg tgcagggtgt
     5821 ccgaggaagt caagctcagc ctgacagccc tagtgctcag ctcgccctca ttgctgccag
     5881 ccagagcttc ctgcagccag gtgggaagat ggtggcagcg gcaaaagcct cagtgccaac
     5941 aattcaggac caggcttcag cgatgcagct gagtcagtgc gcaaagaacc taggcactgc
     6001 cctggccgag ctgcgcactg ctgctcagaa ggctcaggag gcatgtggac ctttggagat
     6061 ggattctgca ctgagtgttg tacaaaatct agagaaagac ctacaggaaa taaaagcagc
     6121 agctcgagat ggcaagctta agcccttacc tggggaaaca atggagaagt gtacccaaga
     6181 tcttggcaac agcaccaaag cggtgagctc tgccatcgcc aagctgctgg gggagattgc
     6241 ccagggcaat gagaactatg caggtattgc agctcgggat gtagctggtg gactaaggtc
     6301 actagcccag gctgcgcggg gtgtggctgc gctgacatca gatcctgcag tgcaggccat
     6361 tgtgcttgac acagccagcg acgttctgga caaggccagc agcctcattg aagaggcaaa
     6421 aaaagcatct ggacacccag gggacccaga aagccagcaa aggcttgctc aggtagctaa
     6481 agcagtgacc caagccctga accgctgtgt cagctgcctg cctggccaaa gagacgtgga
     6541 taatgcccta cgagccgttg gagatgccag caagcgactc ctgagtgact tgcttcctcc
     6601 aagcacgggg acatttcaag aagcacagag ccgattgaat gaagctgcag ctgggttaaa
     6661 tcaggccgcc acagaactgg tgcaagcctc ccgaggaact cctcaggacc tggctcgggc
     6721 ctccggccga tttggacagg acttcagcac cttcctggaa gctggtgtgg agatggccgg
     6781 acaggccccg agccaggagg accgagccca ggtggtgtcc aacctgaagg gcatatccat
     6841 gtcttcaagc aaacttctcc ttgccgctaa ggccttgtcc acagaccctg cttctcccaa
     6901 cctcaagagt cagctggctg cggctgcccg ggcagtgacg gacagcatca accagctcat
     6961 caccatgtgc acccagcagg cacctggcca gaaggagtgt gacaatgcac ttcggcagct
     7021 agagacagtc cgagaactcc tggagaatcc agtccagccc atcaacgaca tgtcctactt
     7081 cggttgcttg gacagtgtca tggagaactc caaggtccta ggtgaggcca tgactggcat
     7141 ctcccaaaat gccaagaatg gaaatctgcc ggagtttgga gatgccattg ccacagcctc
     7201 caaggctctc tgtggcttca cggaggcagc cgcacaggca gcatatctag ttggtgtctc
     7261 tgaccccaac agccaagctg gacagcaagg actggtggaa cccacacagt ttgcccgtgc
     7321 aaaccaggca attcagatgg cctgtcagag tctgggggag cctggctgta cccaggccca
     7381 ggtgttatct gcagccacta ttgtagccaa acacacatct gcattgtgta acagctgtcg
     7441 cctggcttcc gctaggactg ccaatcccac tgccaagcgc cagtttgtac agtcagccaa
     7501 ggaggtggcc aacagtacag ccaatcttgt caagaccatc aaggcactag atggggactt
     7561 cacagaagag aaccgtgccc agtgccgagc agccacagcc cctctgctgg aagctgtgga
     7621 taacctgagt gcctttgcct ccaaccctga gttctccagc gtccctgccc agatcagccc
     7681 tgagggcaga gcagccatgg agcccattgt aatctctgct aagacaatgt tggagagtgc
     7741 tggagggctc atccagacag ctcgggcgtt agcagtcaat ccccgagacc ccccacgttg
     7801 gtctgtgcta gctggccact ctcggactgt ctcagattcc atcaagaaac ttattacaag
     7861 catgagggac aaagccccag ggcagctgga gtgtgagaca gccattgcgg ctctgaacag
     7921 ctgcttgcgg gacctagatc aggcttcgct tgccgctgtc agccagcagc ttgctccccg
     7981 tgaaggaatc tctcaagagg ctttgcacac ccagatgctc actgcagtgc aagaaatttc
     8041 tcatctcatt gagccgctgg ccagcgctgc tcgggctgaa gcctcccagc tgggacacaa
     8101 ggtgtcccaa atggcccagt actttgaacc actcaccctg gctgcagtgg gtgctgcgtc
     8161 taagaccctg agccacccgc agcagatggc acttctggac cagactaaaa cgttggcaga
     8221 gtctgccttg cagttgctat atactgccaa ggaggctggt ggtaacccca agcaagcagc
     8281 acacacccag gaagccctgg aggaggctgt gcagatgatg acagaggccg tagaagacct
     8341 gacgacgacc ctcaacgaag cagccagtgc tgcaggagtc gttggcggca tggtggactc
     8401 tatcactcag gccatcaacc agctagatga aggacctatg ggtgacccag aaggctcatt
     8461 cgtagattac cagacaacca tggtgaggac agccaaggcc attgctgtca ctgttcagga
     8521 gatggtaacc aagtcaaaca ccagccctga agagctaggc cctcttgcca accagctgac
     8581 cagtgactat ggccgactgg cctcacaagc caagcctgca gctgtggctg ctgaaaatga
     8641 agagataggc gctcacatca aacaccgagt acaggagctg ggccatggct gctctgctct
     8701 ggtcaccaag gcaggtgcct tgcagtgtag ccccagtgat gtctacacca agaaggagct
     8761 catagagtgt gcccgcagag tgtcagagaa ggtctcccat gttctggctg cactccaggc
     8821 tgggaatcgt ggtacccagg cctgcattac agcagccagt gctgtgtctg gtatcattgc
     8881 tgacctcgac accaccatca tgttcgctac tgctggcaca cttaaccgtg agggtgctga
     8941 aacttttgct gaccaccggg agggtatctt aaagacagca aaggttcttg tggaggacac
     9001 caaggtccta gtgcagaatg cagctgggag ccaggagaag ttggcacaag ccgcccagtc
     9061 ctccgtggcc accattaccc gcctcgctga tgtggtcaag ctcggtgcag ccagcctagg
     9121 agccgaagac cctgaaactc aggtggtgct gatcaatgca gtaaaggacg tagccaaggc
     9181 cctgggtgac ctcatcagcg ctacgaaggc tgcagcgggc aaagttgggg atgaccctgc
     9241 agtgtggcag ctcaagaact ctgccaaggt gatggtgacc aatgtgacat cattgctcaa
     9301 gacagtgaag gctgtggaag atgaggccac caaaggcaca cgggccctag aggcaaccac
     9361 agagcacata cgtcaggaac tggcggtctt ctgttcccca gagccacctg ccaagacctc
     9421 tacccctgaa gacttcatcc gaatgaccaa gggtattact atggcaacag ccaaagccgt
     9481 tgctgctggc aattcctgtc gacaggaaga tgtcattgcc acagccaatc tgagccgacg
     9541 ggctattgcg gacatgcttc gggcttgcaa ggaagcagct ttccacccag aagtggcgcc
     9601 tgatgtacgg ctccgagccc tgcactatgg ccgggagtgt gccaatggtt acctggaact
     9661 gctggaccac gtgctgctga cccttcagaa gccaaaccca gacctgaagc agcagctaac
     9721 tggacactca aagcgggttg ctggctctgt gactgagctc atccaggctg ctgaagccat
     9781 gaaaggaaca gagtgggtgg acccagagga ccctactgtc attgctgaga atgagctctt
     9841 gggagccgca gccgccatcg aggctgcagc caagaagctg gagcagctga agcccagggc
     9901 caaacccaag gaggcggatg agtccttgaa ctttgaggaa caaatcctag aagctgccaa
     9961 gtccatcgct gcagccacca gtgcactggt aaaggctgcg tcagcagccc agagggagct
    10021 ggtggctcaa ggaaaggtgg gcgccattcc agccaatgca ctggatgatg ggcagtggtc
    10081 gcagggcctc atttccgctg cccgtatggt ggctgcggcc accaacaatc tgtgtgaagc
    10141 agctaacgca gctgtccagg gccatgctag ccaggagaaa ctcatttcct cagccaagca
    10201 agtagctgcc tccacagctc agctcctggt agcttgcaag gtcaaagcag atcaggactc
    10261 tgaggcaatg aaacggctcc aggctgctgg caatgcagtg aagagggcct cagataacct
    10321 ggtaaaggcg gcccagaaag ctgcagcctt cgaagaccag gagaatgaga cggtggtggt
    10381 gaaggagaaa atggttgggg gcattgccca gattatcgca gcacaggaag agatgcttcg
    10441 gaaggaacga gagctggaag aggctcggaa aaagctcgcc cagatccggc agcagcagta
    10501 caagttcttg ccttcagagc ttcgagacga gcacatcgat gggtctacat ctggatctgg
    10561 gaagccgggt tctggtgagg gttctatggt gagcaagggc gaggagctgt tcaccggggt
    10621 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
    10681 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
    10741 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
    10801 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
    10861 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
    10921 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
    10981 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
    11041 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
    11101 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
    11161 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
    11221 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg gggggcccgg
    11281 tggtgcagga ccaggtggtg caggtccagg tggtgcagga ccaggtggtg caggacctgg
    11341 tggtgcaggt ccaggtggtg caggtccagg tggtgcaggt ccaggtggtg ctcatatggt
    11401 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
    11461 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
    11521 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
    11581 ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga
    11641 cttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
    11701 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
    11761 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
    11821 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaag
    11881 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata
    11941 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag
    12001 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
    12061 ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaattaaa tcgataaaat
    12121 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
    12181 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
    12241 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
    12301 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
    12361 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
    12421 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
    12481 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
    12541 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
    12601 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
    12661 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
    12721 gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc
    12781 ccagggacca ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg
    12841 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    12901 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
    12961 cagccatgac ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg
    13021 ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc
    13081 cggaaccctt aatataactt cgtataatgt atgctatacg aagttatcag tactggctta
    13141 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    13201 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    13261 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    13321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    13381 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    13441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    13861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    14041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    14101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    14161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    14221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    14281 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    14341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    14401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    14461 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
    14521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14761 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    14821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    14881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    14941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    15001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    15061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    15121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    15181 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    15241 aagaattgct agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt
    15301 cctccaaatg tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc
    15361 taccctattc cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct
//
