LOCUS       sequence-158766-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2E
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caaacttacg ttgagtgatt aacttggcgt ttagaagttg tctccaatag tttttcatct
       61 ttttgactac ttcttcactt ggaacgttat ccgatttacc acgattttta tcagaacgcg
      121 ttaagacctt attgtctatt gaatcgtctt taaggaaact ttgtggaaca atggcatcga
      181 catcataatc acttaaacga ttaatatcta attcttggtc cacatacatg tctcttccat
      241 tttggagata atagagatag agcttttcat tttgcaattg agtattttca acaggatgct
      301 ctttaagaat ctgacttcct aattctttga taccttcttc gattcgtttc atacgctctc
      361 gcgaattttt ctggcccttt tgagttgtct gattttcacg tgccatttca ataacgatat
      421 tttctggctt atgccgcccc attactttga ccaattcatc aacaactttt acagtctgta
      481 aaataccttt tttaatagca gggctaccag ctaaatttgc aatatgttca tgtaaactat
      541 cgccttgtcc agacacttgt gctttttgaa tgtcttcttt aaatgtcaaa ctatcatcat
      601 ggatcagctg cataaaattg cgattggcaa aaccatctga tttca
//
