LOCUS       sequence-158767-         339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, E RBS: AGGGCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2E
FEATURES             Location/Qualifiers
     source          1..339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(12..46)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 tttctctagt agctagcata atccctagga ctgagctagc catcaggtta acaaaaaagc
       61 aggaatatcc ctatcagtga tagacagaca tccctatcag tgatagagta taaagttaac
      121 tgtgggattg caaaaggagc tcccaactca taaagtcaag tagggcgtta attccatgga
      181 taagaaatac tcaataggct tagctatcgg cacaaatagc gtcggatggg cggtgatcac
      241 tgatgaatat aaggttccgt ctaaaaagtt caaggttctg ggaaatacag accgccacag
      301 tatcaaaaaa aatcttatag gggctctttt atttgacag
//
