LOCUS       sequence-158769-         683 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from aTc-inducible promoter in acsA, F RBS: TGGGCG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas2F
FEATURES             Location/Qualifiers
     source          1..683
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(16..50)
                     /label=BBa_J23113
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.01"
ORIGIN
        1 cctctttctc tagtagctag cataatccct aggactgagc tagccatcag gttaacaaaa
       61 aagcaggaat atccctatca gtgatagaca gacatcccta tcagtgatag agtataaagt
      121 taactgtggg attgcaaaag gagctcccaa ctcataaagt caagttgggc gttaattcca
      181 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
      241 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
      301 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
      361 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
      421 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
      481 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
      541 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
      601 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
      661 tgattaagtt tcgtggtcat ttt
//
