LOCUS       sequence-158770-        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Experimental plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Pulido-Quetglas C, Aparicio-Prat E, Arnan C, Polidori T, Hermoso T, 
            Palumbo E, Ponomarenko J, Guigo R, Johnson R
  TITLE     Scalable Design of Paired CRISPR Guide RNAs for Genomic Deletion.
  JOURNAL   PLoS Comput Biol. 2017 Mar 2;13(3):e1005341. doi: 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar.
  PUBMED    28253259
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2017-03-2"; volume: "13(3):e1005341. doi"; pages: " 
            10.1371/journal.pcbi.1005341. eCollection 2017 Mar"
COMMENT     Sequence Label: pDECKO-mCherry MALAT1_Enhancer.1
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    156..273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_RNA        complement(329..404)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(433..647)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(493..512)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     misc_RNA        complement(654..729)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(751..999)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(809..828)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(979..999)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tgacgggcac cggagccaat tcccactcct ttcaagacct agaaggtcca ttagctgcaa
       61 agattcctct ctgtttaaaa ctttatccat ctttgcaccc gggccccctc gagccggcgc
      121 caaagtggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt tgtaatttgt
      181 ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct ttcccctgca
      241 ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc catttgtctc
      301 aagatctagt tacgccaagc ttaaaaaagc accgactcgg tgccactttt tcaagttgat
      361 aacggactag ccttatttta acttgctatt tctagctcta aaactcctga gctgaggcag
      421 aactgggaaa gagtggtctc atacagaact tataagactc ccaaatccaa agacatttca
      481 cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc
      541 ctgtccctca cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg
      601 acactgggcc cgcgattcct tggagcgggt tgatgacgtc agcgttcaaa aaagcaccga
      661 ctcggtgcca ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttctag
      721 ctctaaaacc gtgtctttac ttgaccaacc ggtgtttcgt cctttccaca agatatataa
      781 agccaagaaa tcgaaatact ttcaagttac ggtaagcata tgatagtcca ttttaaaaca
      841 taattttaaa actgcaaact acccaagaaa ttattacttt ctacgtcacg tattttgtac
      901 taatatcttt gtgtttacag tcaaattaat tctaattatc tctctaacag ccttgtatcg
      961 tatatgcaaa tatgaaggaa tcatgggaaa taggccctct ctgggtccc
//
