LOCUS       sequence-158776-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed from low constituve promoter, c225, in acsA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas7
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgatact gtggcggtct gtatttccca gaaccttgaa ctttttagac ggaaccttat
       61 attcatcagt gatcaccgcc catccgacgc tatttgtgcc gatagctaag cctattgagt
      121 atttcttatc catggaatta atctcctact tgactttatg agttgggagc tccttttgta
      181 atcccacagt taacttttaa caacggtgat aggacttatg tctattacgt cttgttaatt
      241 ttattcctgc ttttttgtta cgaattcctt tgttcgtttc aatgaaggcg aaacgcccct
      301 gttgacccga ttcatccatt tcaatttggg ccacaaaaaa ttccttctgg acaatttccc
      361 cttcctctgt gaaggaaatt tcacccaaag gcgtgacgta ggtacctgca aaaatttcgt
      421 cccgcagttg tcgtcgcaag tccggtagag caagagtttc taagggcgtt ttttcatcga
      481 ggctgctgag ggcttcaaca aaaacttgga tcgccgtaaa agcctgggca ctaaattggg
      541 gcggctcttt ttggttttgt tgaaaatagg cgtcccgaaa cgcgcggtta atctcattat
      601 ctaactcggc actgtaggct tgggccacca acaccccatc acattttgct tggcagacgg
      661 ggaaaatatt agaagtattt aagccgttcc ccccaacaat aatgccgttg taacctaatt
      721 ctcgcagttg cttcaccagg ttgcccgcat cggctgctaa ccccgaa
//
