LOCUS       sequence-158777-         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for constitutive gene expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  TITLE     Lentiviral vector for constitutive gene expression.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: N174-MCS
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..51
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     intron          154..357
                     /label=EF-1-alpha intron A
                     /note="truncated intron upstream of the start codon of 
                     human EF-1-alpha"
     primer_bind     complement(177..197)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg
       61 ccttgggaaa agcgcctccc ctacccggta gaattggatc cacgcgttgt acaatgcatg
      121 cggccgcttc gaagaattcg atcctcacga cacctgaaat ggaagaaaaa aactttgaac
      181 cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga
      241 gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa
      301 ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa
      361 ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga
      421 ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg
      481 cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc
      541 aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag
      601 gccaggcacc
//
