LOCUS       sequence-158778-         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP (truncated to 12 bp) expressed from pJ23119 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas13
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(90..165)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(179..213)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttcg ggcccaagct tcaaaaaaag caccgactcg gtgccacttt ttcaagttga
      121 taacggacta gccttatttt aacttgctat ttctagctct aaaacgaggg cgatgccagc
      181 tagcattata cctaggactg agctagctgt caagaattcc gttattgata caaaattaac
      241 tcaagaaaaa tagtgtgaac taaaagatac agtaggatat tttaatacag caatttcatg
      301 cctatttgac gaatttctaa gtatagtaat aaaaacactt ggagtcagta aaaaaacgac
      361 tgtgttttag tgctttagaa tgcaaagaca ggcgtcactg taagctgaag attttttagc
      421 cgctcttttt ttattttcat atagcctttg agatattttt cctcactatt caaaatatta
      481 tatttactca gtttttaagt aattagcaga caattctaca tcatcatcta gcttattgtg
      541 agtcgattca ttttgtcggg cttcatttgt gctagaagcc cgtccttgtg aaaccaaccg
      601 aactgcagaa cattgccgcg ccagtactgg ataaacctct ggatctactt gaatgcctga
      661 agaaatcatg tcagcacaaa gtttgactgt ttccgctgaa gattggattt gaaactttgc
      721 taattcggct tgttctcttt ccagtagggt agtgctgaaa tctctacagt tgctgcccac
      781 ttgaccaccg aggggcacaa taatggcacc ggaaagagcg taggaatttg aactctcc
//
