LOCUS       sequence-158781-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas14
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(56..85)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(106..181)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(198..232)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 agctggagac cgtttaaact caatgatgat gatgatgatg gtcgacggcg ctattcagat
       61 cctcttctga gatgagtttt tgttcgggcc caagcttcaa aaaaagcacc gactcggtgc
      121 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
      181 cgcgagggcg agggcgagct agcattatac ctaggactga gctagctgtc aagaattccg
      241 ttattgatac aaaattaact caagaaaaat agtgtgaact aaaagataca gtaggatatt
      301 ttaatacagc aatttcatgc ctatttgacg aatttctaag tatagtaata aaaacacttg
      361 gagtcagtaa aaaaacgact gtgttttagt gctttagaat gcaaagacag gcgtcactgt
      421 aagctgaaga ttttttagcc gctctttttt tattttcata tagcctttga gatatttttc
      481 ctcactattc aaaatattat atttactcag tttttaagta attagcagac aattctacat
      541 catcatctag cttattgtga gtcgattcat tttgtcgggc ttcatttgtg ctagaagccc
      601 gtccttgtga aaccaaccga actgcagaac attgccgcgc cagtactgga taaacctctg
      661 gatctacttg aatgcctgaa gaaatcatgt cagcacaaag tttgactgtt tccgctgaag
      721 attggatttg aaactttgct aattcggctt gttctctttc cagtagggta gtgctgaaat
      781 ctctacagtt gctgcccact tgaccaccga ggggcacaat aatggcaccg gaa
//
