LOCUS       sequence-158782-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP (truncated to 15 bp) expressed from pJ23119 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas14
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gtcaagggat ggagaaagca aaatgaataa actgtttaca gcagccctta gtggtctatc
       61 atttatcgtt gttcaacagg caaaaagtga acctgtattt attgattttg attaccagta
      121 tcggtatggt tatgaatata attacgacta tgatgactat acctattact accacgccaa
      181 tgtctacacc tatagcgatg attcggttgt attcccagtc aaagtactcg ttaatgctgc
      241 atgtcaacaa ccacaatacc ccattgtatt aggctctgaa gcagatataa gcaacggtac
      301 agatgtcttg atcgaagaat ctaatgtcat tattgataat gatttcagta caaattgctc
      361 tgcttttaca gaaaaaaatg tcctgctact ctgggaagaa aacgagataa aataggcgtc
      421 ccttctggtt tttctttctg taaagctgta ctgtttaaaa atagatctta aattatagaa
      481 gcactttgac tttattttcg gatccacctt ttcggggaaa tgtgcgcgga acccctattt
      541 gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac
      601 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
      661 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
      721 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
      781 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
      841 gagaatggca aa
//
