LOCUS       sequence-158783-         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP (truncated to 18 bp) expressed from pJ23119 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas15
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(48..123)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(143..177)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 atcctcttct gagatgagtt tttgttcggg cccaagcttc aaaaaaagca ccgactcggt
       61 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      121 aacgcgaggg cgagggcgat gagctagcat tatacctagg actgagctag ctgtcaagaa
      181 ttccgttatt gatacaaaat taactcaaga aaaatagtgt gaactaaaag atacagtagg
      241 atattttaat acagcaattt catgcctatt tgacgaattt ctaagtatag taataaaaac
      301 acttggagtc agtaaaaaaa cgactgtgtt ttagtgcttt agaatgcaaa gacaggcgtc
      361 actgtaagct gaagattttt tagccgctct ttttttattt tcatatagcc tttgagatat
      421 ttttcctcac tattcaaaat attatattta ctcagttttt aagtaattag cagacaattc
      481 tacatcatca tctagcttat tgtgagtcga ttcattttgt cgggcttcat ttgtgctaga
      541 agcccgtcct tgtgaaacca accgaactgc agaacattgc cgcgccagta ctggataaac
      601 ctctggatct acttgaatgc ctgaagaaat catgtcagca caaagtttga ctgtttccgc
      661 tgaagattgg atttgaaact ttgctaattc ggcttgttct ctttccagta gggtagtgct
      721 gaaatctcta cagttgctgc ccacttgacc accgaggggc acaataatgg caccggaaag
      781 agcgtaggaa tttgaactct ccc
//
