LOCUS       sequence-158784-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23117 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas27
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(6..23)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(39..68)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_RNA        complement(89..164)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(186..220)
                     /label=BBa_J23117
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.06"
ORIGIN
        1 actcaatgat gatgatgatg atggtcgacg gcgctattca gatcctcttc tgagatgagt
       61 ttttgttcgg gcccaagctt caaaaaaagc accgactcgg tgccactttt tcaagttgat
      121 aacggactag ccttatttta acttgctatt tctagctcta aaacgcgagg gcgagggcga
      181 tgccagctag cacaatccct aggactgagc tagctgtcaa gaattccgtt attgatacaa
      241 aattaactca agaaaaatag tgtgaactaa aagatacagt aggatatttt aatacagcaa
      301 tttcatgcct atttgacgaa tttctaagta tagtaataaa aacacttgga gtcagtaaaa
      361 aaacgactgt gttttagtgc tttagaatgc aaagacaggc gtcactgtaa gctgaagatt
      421 ttttagccgc tcttttttta ttttcatata gcctttgaga tatttttcct cactattcaa
      481 aatattatat ttactcagtt tttaagtaat tagcagacaa ttctacatca tcatctagct
      541 tattgtgagt cgattcattt tgtcgggctt catttgtgct agaagcccgt ccttgtgaaa
      601 ccaaccgaac tgcagaacat tgccgcgcca gtactggata aacctctgga tctacttgaa
      661 tgcctgaaga aatcatgtca gcacaaagtt tgactgtttc cgctgaagat tggatttgaa
      721 actttgctaa ttcggcttgt tctctttcca gtagggtagt gctgaaatct ctacagttgc
      781 tgcccacttg accaccgagg
//
