LOCUS       sequence-158785-         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas28
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(45..120)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(142..176)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
ORIGIN
        1 ctcttctgag atgagttttt gttcgggccc aagcttcaaa aaaagcaccg actcggtgcc
       61 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
      121 gcgagggcga gggcgatgcc agctagcatt atacctagga ctgagctagc tgtcaggaat
      181 tccgttattg atacaaaatt aactcaagaa aaatagtgtg aactaaaaga tacagtagga
      241 tattttaata cagcaatttc atgcctattt gacgaatttc taagtatagt aataaaaaca
      301 cttggagtca gtaaaaaaac gactgtgttt tagtgcttta gaatgcaaag acaggcgtca
      361 ctgtaagctg aagatttttt agccgctctt tttttatttt catatagcct ttgagatatt
      421 tttcctcact attcaaaata ttatatttac tcagttttta agtaattagc agacaattct
      481 acatcatcat ctagcttatt gtgagtcgat tcattttgtc gggcttcatt tgtgctagaa
      541 gcccgtcctt gtgaaaccaa ccgaactgca gaacattgcc gcgccagtac tggataaacc
      601 tctggatcta cttgaatgcc tgaagaaatc atgtcagcac aaagtttgac tgtttccgct
      661 gaagattgga tttgaaactt tgctaattcg gcttgttctc tttccagtag ggtagtgctg
      721 aaatctctac agttgctgcc cacttgacca ccgaggggca caataatggc accggaaaga
      781 gcgtaggaat ttgaactctc cc
//
