LOCUS       sequence-158787-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from pJ23108 in the NS1 locus with 
            kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas28
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaagcaaaat gaataaactg tttacagcag cccttagtgg tctatcattt atcgttgttc
       61 aacaggcaaa aagtgaacct gtatttattg attttgatta ccagtatcgg tatggttatg
      121 aatataatta cgactatgat gactatacct attactacca cgccaatgtc tacacctata
      181 gcgatgattc ggttgtattc ccagtcaaag tactcgttaa tgctgcatgt caacaaccac
      241 aataccccat tgtattaggc tctgaagcag atataagcaa cggtacagat gtcttgatcg
      301 aagaatctaa tgtcattatt gataatgatt tcagtacaaa ttgctctgct tttacagaaa
      361 aaaatgtcct gctactctgg gaagaaaacg agataaaata ggcgtccctt ctggtttttc
      421 tttctgtaaa gctgtactgt ttaaaaatag atcttaaatt atagaagcac tttgacttta
      481 ttttcggatc caccttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
      541 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
      601 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      661 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      721 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      781 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      841 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaa
//
