LOCUS       sequence-158790-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in 
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas34
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tagtggtcta tcatttatcg ttgttcaaca ggcaaaaagt gaacctgtat ttattgattt
       61 tgattaccag tatcggtatg gttatgaata taattacgac tatgatgact atacctatta
      121 ctaccacgcc aatgtctaca cctatagcga tgattcggtt gtattcccag tcaaagtact
      181 cgttaatgct gcatgtcaac aaccacaata ccccattgta ttaggctctg aagcagatat
      241 aagcaacggt acagatgtct tgatcgaaga atctaatgtc attattgata atgatttcag
      301 tacaaattgc tctgctttta cagaaaaaaa tgtcctgcta ctctgggaag aaaacgagat
      361 aaaataggcg tcccttctgg tttttctttc tgtaaagctg tactgtttaa aaatagatct
      421 taaattatag aagcactttg actttatttt cggatccacc ttttcgggga aatgtgcgcg
      481 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat
      541 tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
      601 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
      661 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
      721 cctattaatt tcccctcgtc aaa
//
