LOCUS       sequence-158794-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dendra2 expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Mohr MA, Argast P, Pantazis P
  TITLE     Labeling cellular structures in vivo using confined primed 
            conversion of photoconvertible fluorescent proteins.
  JOURNAL   Nat Protoc. 2016 Dec;11(12):2419-2431. doi: 10.1038/nprot.2016.134. 
            Epub 2016 Nov 3.
  PUBMED    27809312
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2016.134"; journalName: "Nat Protoc"; date: 
            "2016-12"; volume: "11"; issue: "12"; pages: "2419-2431"
COMMENT     Sequence Label: pGEX6P-1-Dendra2
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..75)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(82..735)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(102..124)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(747..769)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    758..774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(782..788,789..804,805..810))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gcgcatgtcc tccttgatca ggttaattcc cggggtgttc atggatccca ggggcccctg
       61 gaacagaact tccagatccg attttggagg atggtcgcca ccaccaaacg tggcttgcca
      121 gccctgcaaa ggccatgcta tatacttgct ggatttcaag tacttatcaa tttgtgggat
      181 agcttcaata cgttttttaa aacaaactaa ttttgggaac gcatccaggc acattgggtc
      241 catgtataaa acaacatcaa gagcgtcata caacatgaag tcaggatggg ttacatgatc
      301 accatttaaa tatgttttat gacataaacg atcttcgaac attttcagca tttcaggtag
      361 cttgctaaga aaatcaactt tgagagtttc aaagtcttta ctatatgcaa ttctcgaaac
      421 accgtatcta atatccaaaa ccgctccttc aagcattgaa atctctgcac gctcttttgg
      481 acaaccaccc aacatgttgt gcttgtcagc tatataacgt atgatggcca tagactgtgt
      541 taatttaaca tcaccatcaa tataataagg aagattggga aactccaaac ccaattcaaa
      601 ctttttgttt cgccatttat caccttcatc gcgctcatac aaatgctctt catatttttc
      661 ttcaagatat tccaaaagaa gtcgagtggg ttgcacaagg cccttaattt tccaataacc
      721 tagtataggg gacatgaata ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      781 acattatacg agccgatgat taattgtcaa cagctcattt cagaatattt gccagaaccg
      841 ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt gcgccttgag cgacacgaat
      901 tatgcagtga tttacgacct gcacagccat accacagctt ccgatggctg cctgacgcca
      961 gaagcattgg tgcaccgtgc agtcgaataa gctaattcca acgccatcaa a
//
