LOCUS       sequence-158795-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting cpcB expressed from aTc inducible system in pEZ03 in
            the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas35
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaataaac tgtttacagc agcccttagt ggtctatcat ttatcgttgt tcaacaggca
       61 aaaagtgaac ctgtatttat tgattttgat taccagtatc ggtatggtta tgaatataat
      121 tacgactatg atgactatac ctattactac cacgccaatg tctacaccta tagcgatgat
      181 tcggttgtat tcccagtcaa agtactcgtt aatgctgcat gtcaacaacc acaatacccc
      241 attgtattag gctctgaagc agatataagc aacggtacag atgtcttgat cgaagaatct
      301 aatgtcatta ttgataatga tttcagtaca aattgctctg cttttacaga aaaaaatgtc
      361 ctgctactct gggaagaaac gagataaaat aggcgtccct tctggttttt ctttctgtaa
      421 agctgtactg tttaaaaata gatcttaaat tatagaagca ctttgacttt attttcggat
      481 ccaccttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      541 aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg
      601 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
      661 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
      721 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
      781 aagtgagaaa tcaccatgag tgacgactg
//
