LOCUS       sequence-158819-         794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP +172 from TSS; non-template strand; expressed 
            from aTc inducible system in pEZ03 in the NS1 locus with kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas55
FEATURES             Location/Qualifiers
     source          1..794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttaacggctg tctccacagt gttcctgact cctatttacg gagcggctca aacgatgcaa
       61 gatcaaagta atacgatgaa taatgaaaat tcattgggga atagtgcgac tggggtagtc
      121 aaccaaaccg ctgtgtacaa cttaggtcaa agtgccacct acggttttag agggatcgtt
      181 tgtccccgcc cttcctttgt gctcagtggc tctgcaaatg gcaatacatt tgatggggag
      241 agttcaaatt cctacgctct ttccggtgcc attattgtgc ccctcggtgg tcaagtgggc
      301 agcaactgta gagatttcag cactacccta ctggaaagag aacaagccga attagcaaag
      361 tttcaaatcc aatcttcagc ggaaacagtc aaactttgtg ctgacatgat ttcttcaggc
      421 attcaagtag atccagaggt ttatccagta ctggcgcggc aatgttctgc agttcggttg
      481 gtttcacaag gacgggcttc tagcacaaat gaagcccgac aaaatgaatc gactcacaat
      541 aagctagatg atgatgtaga attgtctgct aattacttaa aaactgagta aatataatat
      601 tttgaatagt gaggaaaaat atctcaaagg ctatatgaaa ataaaaaaag agcggctaaa
      661 aaatcttcag cttacagtga cgcctgtctt tgcattctaa agcactaaaa cacagtcgtt
      721 tttttactga ctccaagtgt ttttattact atacttagaa attcgtcaaa taggcatgaa
      781 attgctgtat taaa
//
