LOCUS       sequence-158821-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP +46 from TSS; template strand; expressed from 
            aTc inducible system in pEZ03 in the NS1 locus with kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas56
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cttttaacgg ctgtctccac agtgttcctg actcctattt acggagcggc tcaaacgatg
       61 caagatcaaa gtaatacgat gaataatgaa aattcattgg ggaatagtgc gactggggta
      121 gtcaaccaaa ccgctgtgta caacttaggt caaagtgcca cctacggttt tagagggatc
      181 gtttgtcccc gcccttcctt tgtgctcagt ggctctgcaa atggcaatac atttgatggg
      241 gagagttcaa attcctacgc tctttccggt gccattattg tgcccctcgg tggtcaagtg
      301 ggcagcaact gtagagattt cagcactacc ctactggaaa gagaacaagc cgaattagca
      361 aagtttcaaa tccaatcttc agcggaaaca gtcaaacttt gtgctgacat gatttcttca
      421 ggcattcaag tagatccaga ggtttatcca gtactggcgc ggcaatgttc tgcagttcgg
      481 ttggtttcac aaggacgggc ttctagcaca aatgaagccc gacaaaatga atcgactcac
      541 aataagctag atgatgatgt agaattgtct gctaattact taaaaactga gtaaatataa
      601 tattttgaat agtgaggaaa aatatctcaa aggctatatg aaaataaaaa aagagcggct
      661 aaaaaatctt cagcttacag tgacgcctgt ctttgcattc taaagcacta aaacacagtc
      721 gtttttttac tgactccaag tgtttttatt actatactta gaaattcgtc aaataggcat
      781 gaaattgctg tattaaaata tcctactgta tcttttagtt cacactattt
//
