LOCUS       sequence-158824-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP +111 from TSS; template strand; expressed from 
            aTc inducible system in pEZ03 in the NS1 locus with kanamycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas57
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agcttaatta agtcaaggga tggagaaagc aaaatgaata aactgtttac agcagccctt
       61 agtggtctat catttatcgt tgttcaacag gcaaaaagtg aacctgtatt tattgatttt
      121 gattaccagt atcggtatgg ttatgaatat aattacgact atgatgacta tacctattac
      181 taccacgcca atgtctacac ctatagcgat gattcggttg tattcccagt caaagtactc
      241 gttaatgctg catgtcaaca accacaatac cccattgtat taggctctga agcagatata
      301 agcaacggta cagatgtctt gatcgaagaa tctaatgtca ttattgataa tgatttcagt
      361 acaaattgct ctgcttttac agaaaaaaat gtcctgctac tctgggaaga aaacgagata
      421 aaataggcgt cccttctggt ttttctttct gtaaagctgt actgtttaaa aatagatctt
      481 aaattataga agcactttga ctttattttc ggatccacct tttcggggaa atgtgcgcgg
      541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
      601 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      661 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      721 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      781 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      841 ctgaatccgg tgagaatggc aaa
//
